Key features and details
- Rabbit polyclonal to PLCD4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PLCD4 antibody
See all PLCD4 primary antibodies
DescriptionRabbit polyclonal to PLCD4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human PLCD4 aa 1-250. Two N-terminal tags. (Expressed in E.coli).
MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHA RQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRS NLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWLSDWFQRGDKNQ DGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYK ALTKRAEVQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELID
Database link: Q9BRC7
- IHC-P: Human kidney tissue. WB: Recombinant human PLCD4 protein; Mouse skeletal muscle lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 88 kDa.|
FunctionHydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.
Tissue specificityHighly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells.
Sequence similaritiesContains 1 C2 domain.
Contains 3 EF-hand domains.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
DomainThe PDZ-binding motif mediates the interaction with GRIP1.
The C2 domain mediates pre-localization to the membrane prior to Ca(2+) import and non-selective Ca(2+)-mediated targeting to various cellular membranes.
The PH domain is not a critical determinant of the membrane localization.
Cellular localizationMembrane. Nucleus. Cytoplasm. Endoplasmic reticulum. Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase delta 4 antibody
- 1-phosphatidylinositol 4 antibody
- 1-phosphatidylinositol-4 antibody
Anti-PLCD4 antibody (ab232981) at 2 µg/ml + Mouse skeletal muscle tissue lysate.
Predicted band size: 88 kDa
Anti-PLCD4 antibody (ab232981) at 2 µg + Recombinant human PLCD4 protein.
Predicted band size: 88 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for PLCD4 using ab232981 at 20µg/mL in imunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232981 has not yet been referenced specifically in any publications.