Overview

  • Product name

    Anti-PLCE1 antibody
  • Description

    Rabbit polyclonal to PLCE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PLCE1 aa 161-295.

  • Positive control

    • Human colon tissue.
  • General notes

     This product was previously labelled as Phospholipase C epsilon 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab121476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

Target

  • Function

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.
  • Tissue specificity

    Widely expressed. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen.
  • Involvement in disease

    Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3) [MIM:610725]; also known as early-onset nephrotic syndrome type 3. Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. End-stage kidney disease is observed in steroid-resistant nephrotic syndrome.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 Ras-associating domains.
    Contains 1 Ras-GEF domain.
  • Domain

    The Ras-associating domain 1 is degenerated and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane.
    The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane. Golgi apparatus membrane. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Isoform 1 and isoform 2 associates with Golgi membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase epsilon-1 antibody
    • Pancreas-enriched phospholipase C antibody
    • Phosphoinositide phospholipase C-epsilon-1 antibody
    • Phospholipase C-epsilon-1 antibody
    • PLC-epsilon-1 antibody
    • Plce1 antibody
    • PLCE1_HUMAN antibody
    see all

Images

  • ab121476 staining PLCE1 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

References

ab121476 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121476.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up