Product nameAnti-PLCG 2 antibody
See all PLCG 2 primary antibodies
DescriptionRabbit polyclonal to PLCG 2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PLCG 2 aa 500-550. The exact sequence is proprietary.
Database link: P16885
- WB: Jurkat, RKO and HEK-293T whole cell lysate. IP: Jurkat cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab265582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
50-100 µl/mg lysate
|WB||1/1000. Predicted molecular weight: 148 kDa.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
PLCG 2 was immunoprecipitated from 1 mg Jurkat whole cell lysate using ab265582 at 100 µL per reaction. Western blot was performed on the immunoprecipitate using ab265582 at 1/1000 dilution.
Lane 1: ab265582 IP in Jurkat whole cell lyate.
Lane 2: Control IgG.
All lanes : Anti-PLCG 2 antibody (ab265582) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : RKO whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 148 kDa
Exposure time: 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265582 has not yet been referenced specifically in any publications.