Product nameAnti-PLCG 2 antibody [EPR1403]
See all PLCG 2 primary antibodies
DescriptionRabbit monoclonal [EPR1403] to PLCG 2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human PLCG 2 aa 50-150. The exact sequence is proprietary.
- Ramos and Raji cell lysates, Human kidney tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab109267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol.
|ICC/IF||1/100 - 1/250.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
All lanes : Anti-PLCG 2 antibody [EPR1403] (ab109267) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab109267 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.