Overview

  • Product name

    Anti-PLCG 2 antibody - N-terminal
    See all PLCG 2 primary antibodies
  • Description

    Rabbit polyclonal to PLCG 2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human PLCG 2 (N terminal). The exact sequence is proprietary.
    Database link: P16885

  • Positive control

    • ICC/IF: HeLa cells. WB: Raji whole cell lysate; RAW 264.7 whole cell extract. IHC-P: BT474 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/20000. Predicted molecular weight: 148 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase gamma-2 antibody
    • EC 3.1.4.11 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-gamma-2 antibody
    • Phospholipase C gamma 2 antibody
    • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
    • Phospholipase C-gamma-2 antibody
    • Phospholipase C-IV antibody
    • PLC 2 antibody
    • PLC gamma 2 antibody
    • PLC IV antibody
    • PLC-gamma-2 antibody
    • PLC-IV antibody
    • Plcg2 antibody
    • PLCG2_HUMAN antibody
    see all

Images

  • Paraffin-embedded BT474 xenograft tissue stained for PLCG 2 using ab227129 at 1/100 dilution in immunohistochemical analysis.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PLCG 2 (green) using ab227129 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

  • Anti-PLCG 2 antibody - N-terminal (ab227129) at 1/10000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 148 kDa



    5% SDS-PAGE gel.

  • Anti-PLCG 2 antibody - N-terminal (ab227129) at 1/500 dilution + RAW 264.7 (nouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 148 kDa



    5% SDS-PAGE gel.

References

ab227129 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227129.
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