Key features and details
- Rabbit polyclonal to PLCG 2 (phospho Y1217)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLCG 2 (phospho Y1217) antibody
See all PLCG 2 primary antibodies
DescriptionRabbit polyclonal to PLCG 2 (phospho Y1217)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human PLCG 2 (internal sequence) (phospho Y1217). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P16885
- WB: Pervanadate treated Raji whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 148 kDa.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
All lanes : Anti-PLCG 2 (phospho Y1217) antibody (ab227053) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) treated with 1 mM pervanadate for 30 minutes, whole cell extract
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 148 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227053 has not yet been referenced specifically in any publications.