Product nameAnti-PLCG 2 (phospho Y1217) antibody
See all PLCG 2 primary antibodies
DescriptionRabbit polyclonal to PLCG 2 (phospho Y1217)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human PLCG 2 (internal sequence) (phospho Y1217). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P16885
- WB: Pervanadate treated Raji whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 148 kDa.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
All lanes : Anti-PLCG 2 (phospho Y1217) antibody (ab227053) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) treated with 1 mM pervanadate for 30 minutes, whole cell extract
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 148 kDa
ab227053 has not yet been referenced specifically in any publications.