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    plcg-2-phospho-y1217-antibody-ep1404y-ab52610.pdf

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RabMAb

Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)

  • Datasheet
Reviews (1)Q&A (2)

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Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
  • Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
  • Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)

Key features and details

  • Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)
  • Suitable for: WB, Dot blot
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]
    See all PLCG 2 primary antibodies
  • Description

    Rabbit monoclonal [EP1404Y] to PLCG 2 (phospho Y1217)
  • Host species

    Rabbit
  • Specificity

    ab52610 only detects PLCG2 phosphorylated on Tyrosine 1217.
  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos and Raji cell lysates treated with pervanadate. IHC-P: Human lymph node tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1404Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Dot blot
1/1000.
Notes
WB
1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Dot blot
1/1000.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
  • Target information above from: UniProt accession P16885 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5336 Human
    • Omim: 600220 Human
    • SwissProt: P16885 Human
    • Unigene: 413111 Human
    • Alternative names

      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase gamma-2 antibody
      • EC 3.1.4.11 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-2 antibody
      • Phospholipase C gamma 2 antibody
      • Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody
      • Phospholipase C-gamma-2 antibody
      • Phospholipase C-IV antibody
      • PLC 2 antibody
      • PLC gamma 2 antibody
      • PLC IV antibody
      • PLC-gamma-2 antibody
      • PLC-IV antibody
      • Plcg2 antibody
      • PLCG2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
      Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
      All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/1000 dilution

      Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
      Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
      Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 150 kDa
      Observed band size: 150 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
      Dot Blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)

      Dot blot analysis of PLCG 2 (phospho Y1217) phospho peptide (Lane 1) and PLCG 2 phospho peptide (Lane 2) labelling PLCG 2 (phospho Y1217) with ab52610 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
      Western blot - Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610)
      All lanes : Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y] (ab52610) at 1/50000 dilution

      Lane 1 : Raji cell lysate (untreated)
      Lane 2 : Raji cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 150 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab52610? Please let us know so that we can cite the reference in this datasheet.

    ab52610 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PLCG 2 (phospho Y1217) antibody [EP1404Y]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
    Sample
    Human Tissue sections (Stomach)
    Specification
    Stomach
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Fixative
    Paraformaldehyde
    Read More

    Mr. Rudolf Jung

    Verified customer

    Submitted Mar 30 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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