Anti-PLCG 2 (phospho Y753) antibody (ab75659)
Key features and details
- Rabbit polyclonal to PLCG 2 (phospho Y753)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLCG 2 (phospho Y753) antibody
See all PLCG 2 primary antibodies -
Description
Rabbit polyclonal to PLCG 2 (phospho Y753) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PLCG 2 (phospho Y753).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/200.
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WB |
1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
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Notes |
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ICC/IF
1/100 - 1/200. |
WB
1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa). |
Target
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Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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Database links
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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Alternative names
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
Images
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All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution
Lane 1 : A431 cell extracts, untreated
Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)
Predicted band size: 148 kDa
Observed band size: 148 kDa -
ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab75659 has been referenced in 1 publication.
- Sadeghi L et al. Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32370190