Product nameAnti-PLCG 2 (phospho Y753) antibody
See all PLCG 2 primary antibodies
DescriptionRabbit polyclonal to PLCG 2 (phospho Y753)
Specificityab75659 detects endogenous levels of PLCG 2 only when phosphorylated at tyrosine 753.
Tested applicationsSuitable for: IP, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide derived from human PLCG 2 around the phosphorylation site of tyrosine 753 (S-L-YP-D-V).
- Extract from A431 cells treated with EGF (200ng/ml, 5min).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab75659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|ICC/IF||1/100 - 1/200.|
|WB||1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution
Lane 1 : A431 cell extracts, untreated
Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)
Predicted band size: 148 kDa
Observed band size: 148 kDa
PLCG 2 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to PLCG 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75659.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 148kDa; PLCG 2
ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.
ab75659 has not yet been referenced specifically in any publications.