Key features and details
- Rabbit polyclonal to PLCG 2 (phospho Y759)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLCG 2 (phospho Y759) antibody
See all PLCG 2 primary antibodies
DescriptionRabbit polyclonal to PLCG 2 (phospho Y759)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human PLCG 2 (internal sequence) (phospho Y759). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P16885
- WB: Raji treated with Pervanadate, whole cell extract.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 148 kDa.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
All lanes : Anti-PLCG 2 (phospho Y759) antibody (ab227001) at 1/1000 dilution
Lane 1 : Untreated Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) treated with 1 mM Pervanadate for 30 minutes whole cell extract
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 148 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227001 has not yet been referenced specifically in any publications.