The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
1/50 - 1/100.
Is unsuitable for Flow Cyt.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Mohammad DK et al. Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3?, regulates shuttling, and attenuates both tonic and induced signaling in B cells. Mol Cell Biol33:3214-26 (2013).
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