Key features and details
- Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLCG 2 (phospho Y759) antibody [EP1406Y]
See all PLCG 2 primary antibodies
DescriptionRabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759)
SpecificityThis antibody only detects PLCG 2 phosphorylated on Tyrosine 759.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic phospho peptide corresponding to residues surrounding Tyr759 of human PLCG 2.
- Raji cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab51020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionThe production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity.
- Information by UniProt
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
All lanes : Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) at 1/100000 dilution
Lane 1 : Raji cell lysate, untreated
Lane 2 : Raji cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51020 has been referenced in 1 publication.
- Mohammad DK et al. Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3?, regulates shuttling, and attenuates both tonic and induced signaling in B cells. Mol Cell Biol 33:3214-26 (2013). PubMed: 23754751