Recombinant
RabMAb

Recombinant Anti-PLCL1 antibody [EPR11213] (ab157200)

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Overview

  • Product name

    Anti-PLCL1 antibody [EPR11213]
    See all PLCL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11213] to PLCL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human PLCL1.

  • Positive control

    • Jurkat cell lysate; fetal brain, fetal kidney and fetal lung cell lysates; Jurkat cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11213

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 123 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Involved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as a inhibitor of PPP1C.

    • Tissue specificity

      Expressed in a variety of fetal and adult organs including brain, lung and kidney. Its expression was greatly reduced in small and non-small cell lung carcinoma. Isoform 1 is predominantly expressed in brain.

    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.

    • Post-translational
      modifications

      Phosphorylated by the catalytic subunit of PKA. Phosphorylation of Thr-93 resulted in dissociation of PPP1C from PRIP1.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Inactive phospholipase C-like protein 1 antibody
        • Inactive protein 1 antibody
        • Phospholipase C deleted in lung carcinoma antibody
        • Phospholipase C epsilon antibody
        • Phospholipase C like 1 antibody
        • Phospholipase C related but catalytically inactive protein antibody
        • Phospholipase C related, but catalytically inactive protein antibody
        • Phospholipase C-deleted in lung carcinoma antibody
        • Phospholipase C-related but catalytically inactive protein antibody
        • Phospholipase C-related catalytically antibody
        • PLC-L1 antibody
        • PLCE antibody
        • PLCL antibody
        • PLCL1 antibody
        • PLCL1_HUMAN antibody
        • PLDL1 antibody
        • PRIP antibody
        see all

      Images

      • Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing PLCL1, using ab157200 at 1/10 dilution for IP.
      • Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
        All lanes : Anti-PLCL1 antibody [EPR11213] (ab157200) at 1/10000 dilution

        Lane 1 : Human fetal brain lysate
        Lane 2 : Human fetal kidney lysate
        Lane 3 : Human fetal lung lysate
        Lane 4 : Jurkat lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 123 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Immunofluorescence analysis of Jurkat cells, labeling PLCL1 using ab157200 at a 1/100 dilution.
      • Flow Cytometry - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Flow Cytometry - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Flow cytometry analysis of permeabilized Jurkat cells labeling PLCL1 (red), using ab157200 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

      References

      ab157200 has not yet been referenced specifically in any publications.

      Publishing research using ab157200? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunohistochemistry (Frozen sections) abreview for Anti-PLCL1 antibody [EPR11213]

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Permeabilization
      Yes - 0.01% triton
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

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      Verified customer

      Submitted Sep 16 2016

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