- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PLCL1 antibody [EPR11213]
See all PLCL1 primary antibodies -
DescriptionRabbit monoclonal [EPR11213] to PLCL1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human PLCL1.
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Positive control
- Jurkat cell lysate; fetal brain, fetal kidney and fetal lung cell lysates; Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11213
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 123 kDa. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. |
Target
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FunctionInvolved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as a inhibitor of PPP1C.
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Tissue specificityExpressed in a variety of fetal and adult organs including brain, lung and kidney. Its expression was greatly reduced in small and non-small cell lung carcinoma. Isoform 1 is predominantly expressed in brain.
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Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
Post-translational
modificationsPhosphorylated by the catalytic subunit of PKA. Phosphorylation of Thr-93 resulted in dissociation of PPP1C from PRIP1. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 5334 Human
- Omim: 600597 Human
- SwissProt: Q15111 Human
- Unigene: 153322 Human
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Alternative names
- Inactive phospholipase C-like protein 1 antibody
- Inactive protein 1 antibody
- Phospholipase C deleted in lung carcinoma antibody
see all
Images
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![Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)](https://www.abcam.com/ps/datasheet/images/157/ab157200/PLCL1-Primary-antibodies-ab157200-1.jpg)
Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing PLCL1, using ab157200 at 1/10 dilution for IP. -
![Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)](https://www.abcam.com/ps/datasheet/images/157/ab157200/PLCL1-Primary-antibodies-ab157200-2.jpg)
Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)All lanes : Anti-PLCL1 antibody [EPR11213] (ab157200) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal lung lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)](https://www.abcam.com/ps/datasheet/images/157/ab157200/PLCL1-Primary-antibodies-ab157200-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)Immunofluorescence analysis of Jurkat cells, labeling PLCL1 using ab157200 at a 1/100 dilution. -
![Flow Cytometry - Anti-PLCL1 antibody [EPR11213] (ab157200)](https://www.abcam.com/ps/datasheet/images/157/ab157200/PLCL1-Primary-antibodies-ab157200-4.jpg)
Flow Cytometry - Anti-PLCL1 antibody [EPR11213] (ab157200)Flow cytometry analysis of permeabilized Jurkat cells labeling PLCL1 (red), using ab157200 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)
Protocols
References
ab157200 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"