Recombinant
RabMAb

Recombinant Anti-PLCL1 antibody [EPR11213] (ab157200)

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Overview

  • Product name

    Anti-PLCL1 antibody [EPR11213]
    See all PLCL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11213] to PLCL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human PLCL1.

  • Positive control

    • Jurkat cell lysate; fetal brain, fetal kidney and fetal lung cell lysates; Jurkat cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11213

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 123 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Involved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as a inhibitor of PPP1C.

    • Tissue specificity

      Expressed in a variety of fetal and adult organs including brain, lung and kidney. Its expression was greatly reduced in small and non-small cell lung carcinoma. Isoform 1 is predominantly expressed in brain.

    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.

    • Post-translational
      modifications

      Phosphorylated by the catalytic subunit of PKA. Phosphorylation of Thr-93 resulted in dissociation of PPP1C from PRIP1.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Inactive phospholipase C-like protein 1 antibody
        • Inactive protein 1 antibody
        • Phospholipase C deleted in lung carcinoma antibody
        • Phospholipase C epsilon antibody
        • Phospholipase C like 1 antibody
        • Phospholipase C related but catalytically inactive protein antibody
        • Phospholipase C related, but catalytically inactive protein antibody
        • Phospholipase C-deleted in lung carcinoma antibody
        • Phospholipase C-related but catalytically inactive protein antibody
        • Phospholipase C-related catalytically antibody
        • PLC-L1 antibody
        • PLCE antibody
        • PLCL antibody
        • PLCL1 antibody
        • PLCL1_HUMAN antibody
        • PLDL1 antibody
        • PRIP antibody
        see all

      Images

      • Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing PLCL1, using ab157200 at 1/10 dilution for IP.
      • Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
        All lanes : Anti-PLCL1 antibody [EPR11213] (ab157200) at 1/10000 dilution

        Lane 1 : Human fetal brain lysate
        Lane 2 : Human fetal kidney lysate
        Lane 3 : Human fetal lung lysate
        Lane 4 : Jurkat lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 123 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Immunofluorescence analysis of Jurkat cells, labeling PLCL1 using ab157200 at a 1/100 dilution.
      • Flow Cytometry - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Flow Cytometry - Anti-PLCL1 antibody [EPR11213] (ab157200)
        Flow cytometry analysis of permeabilized Jurkat cells labeling PLCL1 (red), using ab157200 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

      References

      Publishing research using ab157200? Please let us know so that we can cite the reference in this datasheet.

      ab157200 has been referenced in 1 publication.

      • Xiong Z  et al. Tumor Cell "Slimming" Regulates Tumor Progression through PLCL1/UCP1-Mediated Lipid Browning. Adv Sci (Weinh) 6:1801862 (2019). PubMed: 31131187

      Customer reviews and Q&As

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      Immunohistochemistry (Frozen sections) abreview for Anti-PLCL1 antibody [EPR11213]

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Permeabilization
      Yes - 0.01% triton
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 16 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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