Key features and details
- Rabbit polyclonal to PLCL2 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLCL2 antibody - C-terminal
DescriptionRabbit polyclonal to PLCL2 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, and Jurkat whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab199852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 126 kDa.|
FunctionMay play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Cellular localizationCytoplasm. Predominantly localized to perinuclear areas in both myoblast and myotube C2C12 cells.
- Information by UniProt
- FLJ13484 antibody
- Inactive phospholipase C like protein 2 antibody
- Inactive phospholipase C-like protein 2 antibody
All lanes : Anti-PLCL2 antibody - C-terminal (ab199852) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 126 kDa
Exposure time: 3 minutes
Lysates prepared wtih NETN lysis buffer.
Detection of PLCL2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab199852 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab199852 has not yet been referenced specifically in any publications.