Key features and details
- Rabbit polyclonal to PLD2 (phospho Y169)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLD2 (phospho Y169) antibody
See all PLD2 primary antibodies
DescriptionRabbit polyclonal to PLD2 (phospho Y169)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human PLD2 (phospho Y169) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N).
Database link: O14939
- Extracts of Jurkat cells treated with TNF cell lysate. Human brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192804 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).
Incubate membranes diluted antibody in 2.5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4oC with gentle shaking, overnight.
|IHC-P||1/50 - 1/100.|
FunctionMay have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Sequence similaritiesBelongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain.
- Information by UniProt
- Choline phosphatase 2 antibody
- EC 188.8.131.52 antibody
- hPLD 2 antibody
All lanes : Anti-PLD2 (phospho Y169) antibody (ab192804) at 1/500 dilution
Lane 1 : Jurkat cells treated with TNF lysate
Lane 2 : Jurkat cell treated with TNF lysate with Antigen-specific peptide
Predicted band size: 106 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of formalin fixed paraffin embedded human brain tissue labeling PLD2 (phospho Y169) with ab192804 at 1/50 dilution (left) and preincubated with blocking peptide (right)
ab192804 has not yet been referenced specifically in any publications.