Overview

  • Product name

    Anti-PLD2 (phospho Y169) antibody
    See all PLD2 primary antibodies
  • Description

    Rabbit polyclonal to PLD2 (phospho Y169)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PLD2 (phospho Y169) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N).
    Database link: O14939

  • Positive control

    • Extracts of Jurkat cells treated with TNF cell lysate. Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab192804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).

Incubate membranes diluted antibody in 2.5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4oC with gentle shaking, overnight.

IHC-P 1/50 - 1/100.

Target

Images

  • All lanes : Anti-PLD2 (phospho Y169) antibody (ab192804) at 1/500 dilution

    Lane 1 : Jurkat cells treated with TNF lysate
    Lane 2 : Jurkat cell treated with TNF lysate with Antigen-specific peptide

    Predicted band size: 106 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of formalin fixed paraffin embedded human brain tissue labeling PLD2 (phospho Y169) with ab192804 at 1/50 dilution (left) and preincubated with blocking peptide (right)

References

ab192804 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192804.
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