Product nameAnti-PLD2 (phospho Y169) antibody
See all PLD2 primary antibodies
DescriptionRabbit polyclonal to PLD2 (phospho Y169)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human PLD2 (phospho Y169) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N).
Database link: O14939
- Extracts of Jurkat cells treated with TNF cell lysate. Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192804 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).
Incubate membranes diluted antibody in 2.5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4oC with gentle shaking, overnight.
|IHC-P||1/50 - 1/100.|
FunctionMay have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Sequence similaritiesBelongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain.
- Information by UniProt
- Choline phosphatase 2 antibody
- EC 188.8.131.52 antibody
- hPLD 2 antibody
All lanes : Anti-PLD2 (phospho Y169) antibody (ab192804) at 1/500 dilution
Lane 1 : Jurkat cells treated with TNF lysate
Lane 2 : Jurkat cell treated with TNF lysate with Antigen-specific peptide
Predicted band size: 106 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of formalin fixed paraffin embedded human brain tissue labeling PLD2 (phospho Y169) with ab192804 at 1/50 dilution (left) and preincubated with blocking peptide (right)
ab192804 has not yet been referenced specifically in any publications.