Overview

  • Product name

    Anti-PLD2 (phospho Y169) antibody
    See all PLD2 primary antibodies
  • Description

    Rabbit polyclonal to PLD2 (phospho Y169)
  • Host species

    Rabbit
  • Specificity

    ab78907 detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.

  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PLD2.
    Sequence:

    E-N-YP-L-N


    Database link: NP_002654

  • Positive control

    • IHC-P: human brain tissue WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)

Properties

Applications

Our Abpromise guarantee covers the use of ab78907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 106 kDa.
IHC-P 1/50 - 1/100.

Target

Images

  • All lanes : Anti-PLD2 (phospho Y169) antibody (ab78907) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
    Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 106 kDa
    Observed band size: 94 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of PLD2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.

References

ab78907 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab78907.
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