Key features and details
- Rabbit polyclonal to PLD2 (phospho Y169)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLD2 (phospho Y169) antibody
See all PLD2 primary antibodies
DescriptionRabbit polyclonal to PLD2 (phospho Y169)
ab78907 detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: human brain tissue WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab78907 was purified by affinity chromatography using an epitope specific phosphopeptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab78907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 106 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionMay have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Sequence similaritiesBelongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain.
- Information by UniProt
- Choline phosphatase 2 antibody
- EC 220.127.116.11 antibody
- hPLD 2 antibody
All lanes : Anti-PLD2 (phospho Y169) antibody (ab78907) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 106 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of PLD2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
ab78907 has not yet been referenced specifically in any publications.