Product nameAnti-PLD3 antibody
See all PLD3 primary antibodies
DescriptionRabbit polyclonal to PLD3
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
- Fetal heart lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab76433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:62500.
Tissue specificityWidely expressed. Expressed at higher level in brain. Expressed at low level in corpus callosum, suggesting that it is highly expressed in neurons.
Sequence similaritiesBelongs to the phospholipase D family.
Contains 2 PLD phosphodiesterase domains.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Choline phosphatase 3 antibody
- HindIII K4L homolog antibody
- HU K4 antibody
Western blot analysis of Human adult placenta tissue lysate labeling PLD3 with ab76433 at 1.0µg/ml.
Anti-PLD3 antibody (ab76433) at 1 µg/ml + fetal heart lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Gel concentration 12%
ab76433 has not yet been referenced specifically in any publications.