Anti-PLD3 antibody (ab76433)

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Overview

  • Product name

    Anti-PLD3 antibody
    See all PLD3 primary antibodies

  • Description

    Rabbit polyclonal to PLD3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog

  • Immunogen

    Synthetic peptide corresponding to Human PLD3 aa 38-87 (N terminal).
    Sequence:

    WVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

Target

Images

  • Western blot - Anti-PLD3 antibody (ab76433)
    Western blot - Anti-PLD3 antibody (ab76433)
    Western blot analysis of Human adult placenta tissue lysate labeling PLD3 with ab76433 at 1.0µg/ml.
  • Western blot - Anti-PLD3 antibody (ab76433)
    Western blot - Anti-PLD3 antibody (ab76433)
    Anti-PLD3 antibody (ab76433) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Gel concentration 12%

References

ab76433 has not yet been referenced specifically in any publications.

Publishing research using ab76433? Please let us know so that we can cite the reference in this datasheet.

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1-2 of 2 Abreviews

Western blot abreview for Anti-PLD3 antibody

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
melanoma cell line
Blocking step
block ace as blocking agent for 50 minute(s) · Concentration: 100%
Read More

Dr. 令 中野

Verified customer

Submitted Jul 02 2019

Western blot abreview for Anti-PLD3 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Zebrafish Tissue lysate - whole (total larvae, 4 days post fertilization)
Specification
total larvae, 4 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Dr. Evgenia Salta

Verified customer

Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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