Western blot abreview for Anti-PLD3 antibody

Poor
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
melanoma cell line
Blocking step
block ace as blocking agent for 50 minute(s) · Concentration: 100%

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% block ace
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
20 minute(s) and 0 second(s)

Additional data

Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. This antibody has not been tested with this WB/Dog before, and it is possible that it may not be suitable for use.

Dr. 令 中野

Verified customer

Submitted Jul 02 2019

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