Key features and details
- Rabbit polyclonal to PLD6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PLD6 antibody
See all PLD6 primary antibodies
DescriptionRabbit polyclonal to PLD6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human PLD6 aa 1-252.
MGRLSWQVAAAAAVGLALTLEALPWVLRWLRSRRRRPRREALFFPSQVTC TEALLRAPGAELAELPEGCPCGLPHGESALSRLLRALLAARASLDLCLFA FSSPQLGRAVQLLHQRGVRVRVVTDCDYMALNGSQIGLLRKAGIQVRHDQ DPGYMHHKFAIVDKRVLITGSLNWTTQAIQNNRENVLITEDDEYVRLFLE EFERIWEQFNPTKYTFFPPKKSHGSCAPPVSRAGGRLLSWHRTCGTSSES QT
Database link: Q8N2A8
- WB: Mouse kidney, brain and skeletal muscle lysates. IHC-P: Human testis tissue. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionCardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons.
Tissue specificityWidely expressed. Expression is not limites to testis and ovary.
Sequence similaritiesBelongs to the phospholipase D family. MitoPLD/Zucchini subfamily.
Contains 1 PLD phosphodiesterase domain.
DomainIn contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site (PubMed:17028579).
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- Choline phosphatase 6 antibody
- Mitochondrial cardiolipin hydrolase antibody
- Mitochondrial phospholipase antibody
Paraffin-embedded human testis tissue stained for PLD6 using ab237612 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-PLD6 antibody (ab237612) at 1/2000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse skeletal muscle lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PLD6 (green) using ab237612 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237612 has been referenced in 1 publication.
- Zheng Y et al. Establishment of cell lines with porcine spermatogonial stem cell properties. J Anim Sci Biotechnol 11:33 (2020). PubMed: 32308978