Key features and details
- Goat polyclonal to Pleckstrin
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pleckstrin antibody
See all Pleckstrin primary antibodies
DescriptionGoat polyclonal to Pleckstrin
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- Human blood vessel (platelets) and spleen tissue; Human PBMC lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab115514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 40 kDa.|
|IHC-P||Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMajor protein kinase C substrate of platelets.
Sequence similaritiesContains 1 DEP domain.
Contains 2 PH domains.
- Information by UniProt
- FLJ27168 antibody
- OTTHUMP00000159962 antibody
- p47 antibody
ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human vessel (platelets) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-Pleckstrin antibody (ab115514) at 0.05 µg/ml + Human PBMC lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Primary incubation: 1 hour
ab115514 has not yet been referenced specifically in any publications.