Anti-Pleckstrin antibody (ab115514)
Key features and details
- Goat polyclonal to Pleckstrin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pleckstrin antibody
See all Pleckstrin primary antibodies -
Description
Goat polyclonal to Pleckstrin -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide:
EWIKAIQMASRTGK
with a Cysteine residue linker, corresponding to C terminal amino acids 337-350 of Human Pleckstrin (NP_002655.2). -
Positive control
- Human blood vessel (platelets) and spleen tissue; Human PBMC lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 40 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 40 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Major protein kinase C substrate of platelets. -
Sequence similarities
Contains 1 DEP domain.
Contains 2 PH domains. - Information by UniProt
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Database links
- Entrez Gene: 442956 Dog
- Entrez Gene: 5341 Human
- Entrez Gene: 56193 Mouse
- Entrez Gene: 364206 Rat
- Omim: 173570 Human
- SwissProt: Q6Q308 Dog
- SwissProt: P08567 Human
- SwissProt: Q9JHK5 Mouse
see all -
Alternative names
- FLJ27168 antibody
- OTTHUMP00000159962 antibody
- p47 antibody
see all
Images
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ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human vessel (platelets) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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ab115514 at 3.75ug/ml staining Pleckstrin in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-Pleckstrin antibody (ab115514) at 0.05 µg/ml + Human PBMC lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Primary incubation: 1 hour
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab115514 has been referenced in 1 publication.
- Lezirovitz K et al. A rare genomic duplication in 2p14 underlies autosomal dominant hearing loss DFNA58. Hum Mol Genet 29:1520-1536 (2020). PubMed: 32337552