Overview

  • Product name
  • Description
    Goat polyclonal to Pleckstrin
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide: EWIKAIQMASRTGK, corresponding to C terminal amino acids 337-350 of Human Pleckstrin.

  • Positive control
    • WB: Human heart tissue and peripheral blood mononucleocyte lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human Pleckstrin peptide (ab23303).

Target

Images

  • ab17020 at 0.05 µg/ml detecting Pleckstrin from Human PBMC lysate (35µg total protein per lane in RIPA buffer). Primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.

References

This product has been referenced in:
  • Moore SF  et al. Dysfunction of the PI3 kinase/Rap1/integrin a(IIb)ß(3) pathway underlies ex vivo platelet hypoactivity in essential thrombocythemia. Blood 121:1209-19 (2013). Human . Read more (PubMed: 23243278) »
  • Fälker K  et al. Protease-activated receptor 1 (PAR1) signalling desensitization is counteracted via PAR4 signalling in human platelets. Biochem J 436:469-80 (2011). Read more (PubMed: 21391917) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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