Product nameAnti-Plectin antibody - C-terminal
See all Plectin primary antibodies
DescriptionGuinea pig polyclonal to Plectin - C-terminal
Host speciesGuinea pig
SpecificityLocalizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmo- somes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes).
Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human, Xenopus laevis, Fish
Does not react with: Mouse, Cow
Recombinant fragment corresponding to Human Plectin aa 4367-4684 (C terminal).
TEFA DMLSGNAGGF RSRSSSVGSS SSYPISPAVS RTQLASWSDP TEETGPVAGI LDTETLEKVS ITEAMHRNLV DNITGQRLLE AQACTGGIID PSTGERFPVT DAVNKGLVDK IMVDRINLAQ KAFCGFEDPR TKTKMSAAQA LKKGWLYYEA GQRFLEVQYL TGGLIEPDTP GRVPLDEALQ RGTVDARTAQ KLRDVGAYSK YLTCPKTKLK ISYKDALDRS MVEEGTGLRL LEAAAQSTKG YYSPYSVSGS GSTAGSRTGS RTGSRAGSRR GSFDATGSGF SMTFSSSSYS SSGYGRRYAS GSSASLGGPE SAVA
Database link: Q15149
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Our Abpromise guarantee covers the use of ab192706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/16000. Predicted molecular weight: 532 kDa.|
|IHC-P||1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionInterlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
Tissue specificityWidely expressed with highest levels in muscle, heart, placenta and spinal cord.
Involvement in diseaseEpidermolysis bullosa simplex with pyloric atresia
Epidermolysis bullosa simplex, with muscular dystrophy
Epidermolysis bullosa simplex, Ogna type
Limb-girdle muscular dystrophy 2Q
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 33 plectin repeats.
Contains 4 spectrin repeats.
DomainThe N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
modificationsPhosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > hemidesmosome.
- Information by UniProt
- EBS1 antibody
- EBSO antibody
- HD1 antibody
ab192706 has not yet been referenced specifically in any publications.