Overview

  • Product name

    Anti-Plectin antibody - C-terminal
    See all Plectin primary antibodies
  • Description

    Guinea pig polyclonal to Plectin - C-terminal
  • Host species

    Guinea pig
  • Specificity

    Localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmo- somes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes).
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Xenopus laevis, Fish
    Does not react with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Plectin aa 4367-4684 (C terminal).
    Sequence:

    TEFA DMLSGNAGGF RSRSSSVGSS SSYPISPAVS RTQLASWSDP TEETGPVAGI LDTETLEKVS ITEAMHRNLV DNITGQRLLE AQACTGGIID PSTGERFPVT DAVNKGLVDK IMVDRINLAQ KAFCGFEDPR TKTKMSAAQA LKKGWLYYEA GQRFLEVQYL TGGLIEPDTP GRVPLDEALQ RGTVDARTAQ KLRDVGAYSK YLTCPKTKLK ISYKDALDRS MVEEGTGLRL LEAAAQSTKG YYSPYSVSGS GSTAGSRTGS RTGSRAGSRR GSFDATGSGF SMTFSSSSYS SSGYGRRYAS GSSASLGGPE SAVA


    Database link: Q15149

Properties

Applications

Our Abpromise guarantee covers the use of ab192706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/16000. Predicted molecular weight: 532 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
  • Tissue specificity

    Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
  • Involvement in disease

    Epidermolysis bullosa simplex with pyloric atresia
    Epidermolysis bullosa simplex, with muscular dystrophy
    Epidermolysis bullosa simplex, Ogna type
    Limb-girdle muscular dystrophy 2Q
  • Sequence similarities

    Belongs to the plakin or cytolinker family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 33 plectin repeats.
    Contains 4 spectrin repeats.
  • Domain

    The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
  • Post-translational
    modifications

    Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • EBS1 antibody
    • EBSO antibody
    • HD1 antibody
    • Hemidesmosomal protein 1 antibody
    • PCN antibody
    • pleC antibody
    • PLEC_HUMAN antibody
    • PLEC1 antibody
    • PLEC1b antibody
    • Plectin 1 antibody
    • Plectin 1 intermediate filament binding protein 500kDa antibody
    • Plectin 6 antibody
    • Plectin antibody
    • Plectin-1 antibody
    • PLTN antibody
    see all

References

ab192706 has not yet been referenced specifically in any publications.

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