Key features and details
- Rabbit polyclonal to Plectin - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Plectin antibody - C-terminal
See all Plectin primary antibodies
DescriptionRabbit polyclonal to Plectin - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Recombinant fragment within Human Plectin (C terminal). The exact sequence is proprietary.
Database link: Q15149
- WB: MCF7 and MDA-MB-231 whole cell extracts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 532 kDa.|
FunctionInterlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
Tissue specificityWidely expressed with highest levels in muscle, heart, placenta and spinal cord.
Involvement in diseaseEpidermolysis bullosa simplex with pyloric atresia
Epidermolysis bullosa simplex, with muscular dystrophy
Epidermolysis bullosa simplex, Ogna type
Limb-girdle muscular dystrophy 2Q
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 33 plectin repeats.
Contains 4 spectrin repeats.
DomainThe N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
modificationsPhosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > hemidesmosome.
- Information by UniProt
- EBS1 antibody
- EBSO antibody
- HD1 antibody
All lanes : Anti-Plectin antibody - C-terminal (ab229476) at 1/5000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell extracts
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 532 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229476 has not yet been referenced specifically in any publications.