• Product name
    Anti-Plectin antibody [E398P]
    See all Plectin primary antibodies
  • Description
    Rabbit monoclonal [E398P] to Plectin
  • Host species
  • Specificity
    ab32528 recognises plectin, a 500-kDa intermediate filament binding protein.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Plectin (C terminal). The exact sequence is proprietary.

  • Positive control
    • Jurkat whole cell lysate (ab7899), human skeletal muscle
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab32528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 500 kDa (predicted molecular weight: 531 kDa).
IHC-P 1/250.
IP 1/40.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
    • Tissue specificity
      Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
    • Involvement in disease
      Epidermolysis bullosa simplex with pyloric atresia
      Epidermolysis bullosa simplex, with muscular dystrophy
      Epidermolysis bullosa simplex, Ogna type
      Limb-girdle muscular dystrophy 2Q
    • Sequence similarities
      Belongs to the plakin or cytolinker family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 33 plectin repeats.
      Contains 4 spectrin repeats.
    • Domain
      The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
    • Post-translational
      Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • EBS1 antibody
      • EBSO antibody
      • HD1 antibody
      • Hemidesmosomal protein 1 antibody
      • PCN antibody
      • pleC antibody
      • PLEC_HUMAN antibody
      • PLEC1 antibody
      • PLEC1b antibody
      • Plectin 1 antibody
      • Plectin 1 intermediate filament binding protein 500kDa antibody
      • Plectin 6 antibody
      • Plectin antibody
      • Plectin-1 antibody
      • PLTN antibody
      see all


    • Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250
    • Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI


    This product has been referenced in:
    • Kamal AHM  et al. Inflammatory Proteomic Network Analysis of Statin-treated and Lipopolysaccharide-activated Macrophages. Sci Rep 8:164 (2018). WB ; Mouse . Read more (PubMed: 29317699) »
    • Chen X  et al. Plectin-1 Targeted Dual-modality Nanoparticles for Pancreatic Cancer Imaging. EBioMedicine 30:129-137 (2018). WB ; Human . Read more (PubMed: 29574092) »
    See all 21 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Gallbladder)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C

    Abcam user community

    Verified customer

    Submitted Jan 23 2018


    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit monoclonal [E398P] to Plectin (ab32528) is yet to be tested for use by immunocytochemistry and therefore we cannot guarantee it for this purpose. I have read your technical questionnaire and I have a few comments. Plectin is a cytoskeletal protein that has the potential to be extracted when using a permeabilization agent. My concern is that the triton treatment that you are performing is resulting in the loss of this protein or at least a degree of removal of the epitope from your cells. I would like to recommend the use of acetone as a fixative or the omission of triton in your existing protocol. If this does not lead to an improvement in the results that you have been obtaining please get back in touch with me and I will provide you with a free of charge replacement (provided that the antibody was purchased within the past 90 days).

    Read More


    Sign up