Product nameAnti-Plectin antibody [E398P]
See all Plectin primary antibodies
DescriptionRabbit monoclonal [E398P] to Plectin
Specificityab32528 recognises plectin, a 500-kDa intermediate filament binding protein.
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Plectin (C terminal). The exact sequence is proprietary.
- Jurkat cell lysate, human skeletal muscle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab32528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 500 kDa (predicted molecular weight: 531 kDa).|
FunctionInterlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
Tissue specificityWidely expressed with highest levels in muscle, heart, placenta and spinal cord.
Involvement in diseaseEpidermolysis bullosa simplex with pyloric atresia
Epidermolysis bullosa simplex, with muscular dystrophy
Epidermolysis bullosa simplex, Ogna type
Limb-girdle muscular dystrophy 2Q
Sequence similaritiesBelongs to the plakin or cytolinker family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 33 plectin repeats.
Contains 4 spectrin repeats.
DomainThe N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
modificationsPhosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > hemidesmosome.
- Information by UniProt
- EBS1 antibody
- EBSO antibody
- HD1 antibody
This product has been referenced in:
- Kamal AHM et al. Inflammatory Proteomic Network Analysis of Statin-treated and Lipopolysaccharide-activated Macrophages. Sci Rep 8:164 (2018). WB ; Mouse . Read more (PubMed: 29317699) »
- Chen X et al. Plectin-1 Targeted Dual-modality Nanoparticles for Pancreatic Cancer Imaging. EBioMedicine 30:129-137 (2018). WB ; Human . Read more (PubMed: 29574092) »