• Product name
    Anti-Pleiotrophin antibody
    See all Pleiotrophin primary antibodies
  • Description
    Rabbit polyclonal to Pleiotrophin
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Chicken, Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Pleiotrophin.

  • Positive control
    • This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section.



Our Abpromise guarantee covers the use of ab95391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Heparin binding mitogenic protein. Has neurite extension activity.
  • Tissue specificity
    Osteoblast and brain.
  • Sequence similarities
    Belongs to the pleiotrophin family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • HARP antibody
    • HB-GAM antibody
    • HBBM antibody
    • HBGAM antibody
    • HBGF-8 antibody
    • HBGF8 antibody
    • HBNF antibody
    • HBNF-1 antibody
    • HBNF1 antibody
    • heparin affin regulatory protein antibody
    • Heparin binding growth associated molecule antibody
    • Heparin binding growth factor 8 antibody
    • Heparin binding neurite outgrowth promoting factor 1 antibody
    • Heparin-binding brain mitogen antibody
    • Heparin-binding growth factor 8 antibody
    • Heparin-binding growth-associated molecule antibody
    • heparin-binding neurite outgrowth promoting factor antibody
    • Heparin-binding neurite outgrowth-promoting factor 1 antibody
    • NEGF1 antibody
    • Neurite growth promoting factor 1 antibody
    • Neurite outgrowth-promoting factor, heparin-binding antibody
    • OSF-1 antibody
    • OSF1 antibody
    • Osteoblast-specific factor 1 antibody
    • pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) antibody
    • Pleiotrophin antibody
    • PTN antibody
    • PTN_HUMAN antibody
    see all


  • IHC image of Pleiotrophin staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95391, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab95391 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95391, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Zhu X  et al. The effects of pleiotrophin in proliferative diabetic retinopathy. PLoS One 10:e0115523 (2015). IHC-FoFr, ICC/IF ; Human . Read more (PubMed: 25617851) »
  • Lee JY  et al. Chicken pleiotrophin: regulation of tissue specific expression by estrogen in the oviduct and distinct expression pattern in the ovarian carcinomas. PLoS One 7:e34215 (2012). IHC-P ; Chicken . Read more (PubMed: 22496782) »

See all 2 Publications for this product

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