Synthetic peptide within Human Pleiotrophin aa 125-154 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P21246
Our Abpromise guarantee covers the use of ab170606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 19 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Flow cytometric analysis of U251 cells labeling Pleiotrophin with ab170606 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemial analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling Pleiotrophin with ab170606 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab170606 has not yet been referenced specifically in any publications.