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Goat polyclonal to Pleiotrophin - C-terminal
Mouse, HumanPredicted to work with:
Rat, Chicken, Cow, Pig
Synthetic peptide corresponding to Human Pleiotrophin aa 158-168 (C terminal) (Cysteine residue). NP_002816.1.
Database link: P21246
- WB: Mouse fetal brain lysate.
IHC-P: Human prostate tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab223674 purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18.9 kDa).
||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heparin binding mitogenic protein. Has neurite extension activity.
Osteoblast and brain.
Belongs to the pleiotrophin family.
Information by UniProt
- HARP antibody
- HB-GAM antibody
- HBBM antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pleiotrophin antibody - C-terminal (ab223674)
Paraffin-embedded human prostate tissue stained for Pleiotrophin using ab223674 at 3.8 µg/ml in immunohistochemical analysis, followed by AP-staining.
Western blot - Anti-Pleiotrophin antibody - C-terminal (ab223674)
Anti-Pleiotrophin antibody - C-terminal (ab223674) at 0.1 µg/ml + mouse fetal brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.Predicted band size:
18.9 kDaObserved band size:
18 kDa (why is the actual band size different from the predicted?
Primary incubation was 1 hour.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"