Key features and details
- Rabbit polyclonal to PLEK2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLEK2 antibody
DescriptionRabbit polyclonal to PLEK2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment, corresponding to amino acids 237-346 of Human PLEK2 (Q9NYT0).
- Human liver tissue and lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Retrieval buffer pH 6.|
FunctionMay help orchestrate cytoskeletal arrangement. Contribute to lamellipodia formation.
Sequence similaritiesContains 1 DEP domain.
Contains 2 PH domains.
Cellular localizationCell projection > lamellipodium membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- Pleckstrin 2 (mouse) homolog antibody
- Pleckstrin 2 antibody
- Pleckstrin-2 antibody
All lanes : Anti-PLEK2 antibody (ab121131) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab121131 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121131 at 1/50 dilution staining PLEK2 in Paraffin-emebedded Human liver tissue by Immunohistochemistry. Note: strong cytoplasmic positivity in hepatocytes.
ab121131 has not yet been referenced specifically in any publications.