Overview

  • Product name
    Anti-PLEKHA4 antibody - N-terminal
  • Description
    Rabbit polyclonal to PLEKHA4 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PLEKHA4 aa 28-58 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9H4M7

  • Positive control
    • K562 cell line lysate; Human liver tissue; K562 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab170537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 85 kDa.

Target

  • Function
    Binds specifically to phosphatidylinositol-3-phosphate (PtdIns3P), but not to other phosphoinositides.
  • Tissue specificity
    Highly expressed in melanoma. Detected at low levels in heart, skeletal muscle, kidney, liver and small intestine.
  • Sequence similarities
    Contains 1 PH domain.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410005C22Rik antibody
    • C88169 antibody
    • MGC72599 antibody
    • PEPP-1 antibody
    • PEPP1 antibody
    • PH domain-containing family A member 4 antibody
    • Phosphoinositol 3 phosphate binding protein 1 antibody
    • Phosphoinositol 3-phosphate-binding protein 1 antibody
    • PKHA4_HUMAN antibody
    • Pleckstrin homology domain containing family A member 4 antibody
    • Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 antibody
    • Pleckstrin homology domain-containing family A member 4 antibody
    • PLEKHA4 antibody
    see all

Images

  • Anti-PLEKHA4 antibody - N-terminal (ab170537) at 1/100 dilution + K562 cell line lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 85 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labeling PLEKHA4 with ab170537 at 1/10 dilution. Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.

  • Flow cytometric analysis of K562 cells (right histogram) labeling PLEKHA4 with ab170537 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

References

ab170537 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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