Anti-PLEKHG4 antibody (ab137898)

Reviews (1)Q&A (1)References (2)
Western blot - Anti-PLEKHG4 antibody (ab137898)

    Key features and details

    • Rabbit polyclonal to PLEKHG4
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-PLEKHG4 antibody
      See all PLEKHG4 primary antibodies

    • Description

      Rabbit polyclonal to PLEKHG4

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Synthetic peptide derived from an internal region of Human PLEKHG4.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at -20ºC.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab137898 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/500 - 1/1000. Predicted molecular weight: 131 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 131 kDa.

    Target

    • Function

      Possible role in intracellular signaling and cytoskeleton dynamics at the Golgi.

    • Tissue specificity

      Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons (at protein level). Widely expressed at low levels. More strongly expressed in testis and pancreas.

    • Sequence similarities

      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARHGEF44 antibody
        • DKFZp434I216 antibody
        • PH domain containing family G member 4 antibody
        • PH domain-containing family G member 4 antibody
        • PKHG4_HUMAN antibody
        • Pleckstrin homology and RhoGEF domain containing G4 antibody
        • Pleckstrin homology domain containing family G (with RhoGef domain) member 4 antibody
        • Pleckstrin homology domain containing family G member 4 antibody
        • Pleckstrin homology domain-containing family G member 4 antibody
        • PLEKHG 4 antibody
        • PLEKHG4 antibody
        • PRTPHN 1 antibody
        • PRTPHN1 antibody
        • Puratrophin 1 antibody
        • Puratrophin-1 antibody
        • Puratrophin1 antibody
        • Purkinje cell atrophy associated protein 1 antibody
        • Purkinje cell atrophy-associated protein 1 antibody
        • SCA 4 antibody
        • SCA4 antibody
        see all

      Images

      • Western blot - Anti-PLEKHG4 antibody (ab137898)
        Western blot - Anti-PLEKHG4 antibody (ab137898)
        Anti-PLEKHG4 antibody (ab137898) at 1/500 dilution + HUVEC cell lysate at 30 µg

        Predicted band size: 131 kDa

      References (2)

      Publishing research using ab137898? Please let us know so that we can cite the reference in this datasheet.

      ab137898 has been referenced in 2 publications.

      • Takenaka N  et al. Rac1 Activation Caused by Membrane Translocation of a Guanine Nucleotide Exchange Factor in Akt2-Mediated Insulin Signaling in Mouse Skeletal Muscle. PLoS One 11:e0155292 (2016). PubMed: 27163697
      • Gupta M  et al. Plekhg4 is a novel Dbl family guanine nucleotide exchange factor protein for rho family GTPases. J Biol Chem 288:14522-30 (2013). IHC-P ; Mouse . PubMed: 23572525

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-PLEKHG4 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (testis and muscle)
      Loading amount
      200 µg
      Specification
      testis and muscle
      Gel Running Conditions
      Reduced Denaturing (8)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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      Abcam user community

      Verified customer

      Submitted Mar 29 2013

      Question

      will test ab137898 in mouse

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      Answer

      Thank you for your phone call.

      DISCOUNT CODE: xxx
      Expiration date: xxx
      Value: xxx

      I am very pleased to hear you would like to accept our offer and test ab137898 in mouse. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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