Anti-PLEKHG4 antibody (ab137898)

Reviews (1)Q&A (1)References (2)

Overview

  • Product name

    Anti-PLEKHG4 antibody
    See all PLEKHG4 primary antibodies

  • Description

    Rabbit polyclonal to PLEKHG4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide derived from an internal region of Human PLEKHG4.

  • Positive control

    • HUVEC cell lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 23572525
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.

Target

  • Function

    Possible role in intracellular signaling and cytoskeleton dynamics at the Golgi.

  • Tissue specificity

    Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons (at protein level). Widely expressed at low levels. More strongly expressed in testis and pancreas.

  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • ARHGEF44 antibody
      • DKFZp434I216 antibody
      • PH domain containing family G member 4 antibody
      • PH domain-containing family G member 4 antibody
      • PKHG4_HUMAN antibody
      • Pleckstrin homology and RhoGEF domain containing G4 antibody
      • Pleckstrin homology domain containing family G (with RhoGef domain) member 4 antibody
      • Pleckstrin homology domain containing family G member 4 antibody
      • Pleckstrin homology domain-containing family G member 4 antibody
      • PLEKHG 4 antibody
      • PLEKHG4 antibody
      • PRTPHN 1 antibody
      • PRTPHN1 antibody
      • Puratrophin 1 antibody
      • Puratrophin-1 antibody
      • Puratrophin1 antibody
      • Purkinje cell atrophy associated protein 1 antibody
      • Purkinje cell atrophy-associated protein 1 antibody
      • SCA 4 antibody
      • SCA4 antibody
      see all

    Images

    • Western blot - Anti-PLEKHG4 antibody (ab137898)
      Western blot - Anti-PLEKHG4 antibody (ab137898)
      Anti-PLEKHG4 antibody (ab137898) at 1/500 dilution + HUVEC cell lysate at 30 µg

      Predicted band size: 131 kDa

    References

    This product has been referenced in:

    • Takenaka N  et al. Rac1 Activation Caused by Membrane Translocation of a Guanine Nucleotide Exchange Factor in Akt2-Mediated Insulin Signaling in Mouse Skeletal Muscle. PLoS One 11:e0155292 (2016). Read more (PubMed: 27163697) »
    • Gupta M  et al. Plekhg4 is a novel Dbl family guanine nucleotide exchange factor protein for rho family GTPases. J Biol Chem 288:14522-30 (2013). IHC-P ; Mouse . Read more (PubMed: 23572525) »
    See all 2 Publications for this product

    Publishing research using ab137898? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PLEKHG4 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (testis and muscle)
    Loading amount
    200 µg
    Specification
    testis and muscle
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 29 2013

    Question

    will test ab137898 in mouse

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    Answer

    Thank you for your phone call.

    DISCOUNT CODE: xxx
    Expiration date: xxx
    Value: xxx

    I am very pleased to hear you would like to accept our offer and test ab137898 in mouse. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
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    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

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