Overview

  • Product name

  • Description

    Rabbit polyclonal to PLEKHG4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human PLEKHG4.

  • Positive control

    • HUVEC cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 23572525
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.

Target

  • Function

    Possible role in intracellular signaling and cytoskeleton dynamics at the Golgi.
  • Tissue specificity

    Expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. Isoform 1 and isoform 3 are strongly expressed in Purkinje cells and to a lower extent in other neurons (at protein level). Widely expressed at low levels. More strongly expressed in testis and pancreas.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGEF44 antibody
    • DKFZp434I216 antibody
    • PH domain containing family G member 4 antibody
    • PH domain-containing family G member 4 antibody
    • PKHG4_HUMAN antibody
    • Pleckstrin homology and RhoGEF domain containing G4 antibody
    • Pleckstrin homology domain containing family G (with RhoGef domain) member 4 antibody
    • Pleckstrin homology domain containing family G member 4 antibody
    • Pleckstrin homology domain-containing family G member 4 antibody
    • PLEKHG 4 antibody
    • PLEKHG4 antibody
    • PRTPHN 1 antibody
    • PRTPHN1 antibody
    • Puratrophin 1 antibody
    • Puratrophin-1 antibody
    • Puratrophin1 antibody
    • Purkinje cell atrophy associated protein 1 antibody
    • Purkinje cell atrophy-associated protein 1 antibody
    • SCA 4 antibody
    • SCA4 antibody
    see all

Images

  • Anti-PLEKHG4 antibody (ab137898) at 1/500 dilution + HUVEC cell lysate at 30 µg

    Predicted band size: 131 kDa

References

This product has been referenced in:

  • Takenaka N  et al. Rac1 Activation Caused by Membrane Translocation of a Guanine Nucleotide Exchange Factor in Akt2-Mediated Insulin Signaling in Mouse Skeletal Muscle. PLoS One 11:e0155292 (2016). Read more (PubMed: 27163697) »
  • Gupta M  et al. Plekhg4 is a novel Dbl family guanine nucleotide exchange factor protein for rho family GTPases. J Biol Chem 288:14522-30 (2013). IHC-P ; Mouse . Read more (PubMed: 23572525) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (testis and muscle)
Loading amount
200 µg
Specification
testis and muscle
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 29 2013

Question
Answer

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DISCOUNT CODE: xxx
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Value: xxx

I am very pleased to hear you would like to accept our offer and test ab137898 in mouse. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
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