Anti-PLEKHH1 antibody - C-terminal (ab171193)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-PLEKHH1 antibody - C-terminal
See all PLEKHH1 primary antibodies -
DescriptionRabbit polyclonal to PLEKHH1 - C-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human PLEKHH1 aa 1089-1118 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9ULM0 -
Positive control
- HepG2 cell lysate; Human brain tissue; HepG2 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab171193 is purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab171193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/100 - 1/500. Predicted molecular weight: 151 kDa. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Cellular localizationCytoskeleton
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Database links
- Entrez Gene: 57475 Human
- SwissProt: Q9ULM0 Human
- Unigene: 594236 Human
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Alternative names
- KIAA1200 antibody
- PH domain containing family H member 1 antibody
- Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 antibody
- pleckstrin homology domain-containing family H member 1 antibody
Images
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Western blot - Anti-PLEKHH1 antibody - C-terminal (ab171193)Anti-PLEKHH1 antibody - C-terminal (ab171193) at 1/100 dilution + HepG2 cell lysate at 35 µg
Predicted band size: 151 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHH1 antibody - C-terminal (ab171193)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling PLEKHH1 with ab171193 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow Cytometry - Anti-PLEKHH1 antibody - C-terminal (ab171193)Flow cytometric analysis of K562 cells labeling PLEKHH1 with ab171193 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Datasheets and documents
References
ab171193 has not yet been referenced specifically in any publications.
