Overview

  • Product name
    Anti-PLEKHH1 antibody - C-terminal
    See all PLEKHH1 primary antibodies
  • Description
    Rabbit polyclonal to PLEKHH1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PLEKHH1 aa 1089-1118 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9ULM0

  • Positive control
    • HepG2 cell lysate; Human brain tissue; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171193 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 151 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Cellular localization
    Cytoskeleton
  • Database links
  • Alternative names
    • KIAA1200 antibody
    • PH domain containing family H member 1 antibody
    • Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 antibody
    • pleckstrin homology domain-containing family H member 1 antibody

Images

  • Anti-PLEKHH1 antibody - C-terminal (ab171193) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 151 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling PLEKHH1 with ab171193 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells labeling PLEKHH1 with ab171193 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab171193 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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