Anti-PLEKHH1 antibody - C-terminal (ab171193)

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Overview

  • Product name
    Anti-PLEKHH1 antibody - C-terminal
    See all PLEKHH1 primary antibodies
  • Description
    Rabbit polyclonal to PLEKHH1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PLEKHH1 aa 1089-1118 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9ULM0

  • Positive control
    • HepG2 cell lysate; Human brain tissue; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171193 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 151 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Cellular localization
    Cytoskeleton
  • Database links
    • Alternative names
      • KIAA1200 antibody
      • PH domain containing family H member 1 antibody
      • Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 antibody
      • pleckstrin homology domain-containing family H member 1 antibody

    Images

    • Western blot - Anti-PLEKHH1 antibody - C-terminal (ab171193)
      Western blot - Anti-PLEKHH1 antibody - C-terminal (ab171193)
      Anti-PLEKHH1 antibody - C-terminal (ab171193) at 1/100 dilution + HepG2 cell lysate at 35 µg

      Predicted band size: 151 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHH1 antibody - C-terminal (ab171193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLEKHH1 antibody - C-terminal (ab171193)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling PLEKHH1 with ab171193 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

    • Flow Cytometry - Anti-PLEKHH1 antibody - C-terminal (ab171193)
      Flow Cytometry - Anti-PLEKHH1 antibody - C-terminal (ab171193)

      Flow cytometric analysis of K562 cells labeling PLEKHH1 with ab171193 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

    References

    ab171193 has not yet been referenced specifically in any publications.

    Publishing research using ab171193? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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