Anti-PLEKHM1 antibody (ab204437)
Key features and details
- Rabbit polyclonal to PLEKHM1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLEKHM1 antibody -
Description
Rabbit polyclonal to PLEKHM1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human PLEKHM1 aa 850-950.
Database link: Q9Y4G2 -
Positive control
- IHC-P: Human Fallopian tube, cerebral cortex, small intestine, placenta tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Involved in vesicular transport in the osteoclast (By similarity). May have a role in sialyl-lex-mediated transduction of apoptotic signals. -
Tissue specificity
Expressed in placenta, liver, prostate, thymus, spleen, ovary, colon, colon carcinoma and peripheral blood lymphocytes (PBL). Weakly expressed in brain, lung, kidney, and testis. No expression in heart, skeletal muscle, pancreas and small intestine. Predominantly expressed in the breast carcinoma cell line MCF-7. -
Involvement in disease
Defects in PLEKHM1 are the cause of osteopetrosis autosomal recessive type 6 (OPTB6) [MIM:611497]; also known as autosomal recessive osteopetrosis intermediate form. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. -
Sequence similarities
Contains 2 PH domains.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 RUN domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9842 Human
- Entrez Gene: 353047 Mouse
- Entrez Gene: 303584 Rat
- Omim: 611466 Human
- SwissProt: Q9Y4G2 Human
- SwissProt: Q7TSI1 Mouse
- SwissProt: Q5PQS0 Rat
- Unigene: 514242 Human
see all -
Alternative names
- 162 kDa adapter protein antibody
- AP162 antibody
- PH domain-containing family M member 1 antibody
see all
Images
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Paraffin embedded human Fallopian tube tissue stained for PLEKHM1 using ab204437 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Immunofluorescent analysis of U-2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling PLEKHM1 with ab204437 at 2 μg/ml (green).
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Paraffin embedded human small intestine tissue stained for PLEKHM1 using ab204437 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human placenta tissue stained for PLEKHM1 using ab204437 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human cerebral cortex tissue stained for PLEKHM1 using ab204437 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204437 has not yet been referenced specifically in any publications.