Overview

  • Product name

    Anti-PLEKHM2 antibody
  • Description

    Rabbit polyclonal to PLEKHM2
  • Host species

    Rabbit
  • Specificity

    ab91581 is specific for PLEKHM2 and will not recognize the other PLEKHM proteins.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide from near the carboxy terminus of Human PLEKHM2 (CAH72018).

  • Positive control

    • Rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 169 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      PLEKHM2, also known as SKIP, is a member of the M family of Pleckstrin homology domain-containing proteins. While little is known of PLEKHM2, a recent study examining differential gene expression in human hematopoietic stem cells has shown it to be specifically expressed in stem cells, suggesting that PLEKHM2 may play a role in erythroid commitment and development. Other studies have shown that PLEKHM2 is required for interaction with the Salmonella virulence factor SifA for Salmonella pathogenesis, suggesting that PLEKHM2 has cellular roles other than in the developing embryo.
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • KIAA0842 antibody
      • Novel RUN and PH domain containing protein antibody
      • PH domain containing family M member 2 antibody
      • Pleckstrin homology domain containing family M (with RUN domain) member 2 antibody
      • Pleckstrin homology domain containing family M member 2 antibody
      • PLEKHM 2 antibody
      • RP11 169K16.1 antibody
      • Salmonella induced filaments A and kinesin interacting protein antibody
      • SifA (Salmonella induced filaments A) and kinesin interacting protein antibody
      • SifA and kinesin interacting protein antibody
      • SKIP antibody
      see all

    Images

    • All lanes : Anti-PLEKHM2 antibody (ab91581) at 0.5 µg/ml

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 169 kDa

    • Immunofluorescence of PLEKHM2 in Human Brain cells using ab91581 at 20 ug/ml.

    References

    ab91581 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your inquiry.
    The band size of proteins on a WB is dependent o0n many factors like percentage of the gel and pH of the puffers, etc.
    Especially big protein are difficult to measure precisely on gels since the marker bands are also affected by these factors and might be affected differently form the analyzed protein.
    PLEKHM2 is also modified on some amino acid residues. This will add to the molecular weight.
    The WB images shows that the band disappears when the antibody is pre-incubated with the blocking peptide. This indicates that the band is specific.
    I hope this information is helpful and wish you good luck with your research.

    Read More

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