Anti-Plexin A1 antibody (ab23391)
- Datasheet
- References (8)
- Protocols
Overview
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Product nameAnti-Plexin A1 antibody
See all Plexin A1 primary antibodies -
DescriptionRabbit polyclonal to Plexin A1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISA, IP, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human Plexin A1 aa 182-200.
Sequence:RLMANEEDADMFGFVYQDE
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Positive control
- Cos 7 cells transfected with mouse Plexin A1 or neonatal rat brain.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab23391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 195 kDa. | |
| ELISA | 1/2000. | |
| IP | 1/50 - 1/200. | |
| ICC/IF | 1/200. (see Abreview). |
Target
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RelevanceFunction: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain.
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Cellular localizationCell Membrane
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Database links
- Entrez Gene: 5361 Human
- Entrez Gene: 18844 Mouse
- NCBI: NP_115618 Human
- Omim: 601055 Human
- SwissProt: Q9UIW2 Human
- SwissProt: P70206 Mouse
- Unigene: 432329 Human
- Unigene: 549421 Human
see all -
Alternative names
- NOV antibody
- NOV/plexin A1 protein antibody
- NOVP antibody
see all
Images
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Western blot - Anti-Plexin A1 antibody (ab23391)Western blots showing Cos-7 cells transfected with mouse myc-tagged Plexin-A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from myc-tagged Plexin-A1 transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (ab23391; lane 5). These blots were probed with either the affinity purified anti-Plexin-A1 (ab23391; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Delloye-Bourgeois C et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci 18:36-45 (2015). Read more (PubMed: 25485759) »
- Hernandez-Enriquez B et al. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing. Genes Dev 29:2617-32 (2015). Read more (PubMed: 26680304) »
