Anti-Plexin A1 antibody (ab23391)
Key features and details
- Rabbit polyclonal to Plexin A1
- Suitable for: IP, ELISA, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-Plexin A1 antibody
See all Plexin A1 primary antibodies -
Description
Rabbit polyclonal to Plexin A1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Plexin A1 aa 182-200.
Sequence:RLMANEEDADMFGFVYQDE
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
| WB |
1/1000. Predicted molecular weight: 195 kDa.
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| Notes |
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IP
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. See Abreview. |
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WB
1/1000. Predicted molecular weight: 195 kDa. |
Target
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Relevance
Function: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 5361 Human
- Entrez Gene: 18844 Mouse
- NCBI: NP_115618 Human
- Omim: 601055 Human
- SwissProt: Q9UIW2 Human
- SwissProt: P70206 Mouse
- Unigene: 432329 Human
- Unigene: 3789 Mouse
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Alternative names
- NOV antibody
- NOV/plexin A1 protein antibody
- NOVP antibody
see all
Images
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Western blot - Anti-Plexin A1 antibody (ab23391)Western blots showing Cos-7 cells transfected with mouse myc-tagged Plexin-A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from myc-tagged Plexin-A1 transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (ab23391; lane 5). These blots were probed with either the affinity purified anti-Plexin-A1 (ab23391; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Datasheets and documents
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Datasheet download
References (12)
ab23391 has been referenced in 12 publications.
- Smirlis D et al. SILAC-based quantitative proteomics reveals pleiotropic, phenotypic modulation in primary murine macrophages infected with the protozoan pathogen Leishmania donovani. J Proteomics 213:103617 (2020). PubMed: 31846769
- Gamper C et al. Functionalized Tobacco Mosaic Virus Coat Protein Monomers and Oligomers as Nanocarriers for Anti-Cancer Peptides. Cancers (Basel) 11:N/A (2019). PubMed: 31652529
- Binamé F et al. Disruption of Sema3A/Plexin-A1 inhibitory signalling in oligodendrocytes as a therapeutic strategy to promote remyelination. EMBO Mol Med 11:e10378 (2019). PubMed: 31566924
- Wang N et al. The Sema3A receptor Plexin-A1 suppresses supernumerary axons through Rap1 GTPases. Sci Rep 8:15647 (2018). PubMed: 30353093
- Delloye-Bourgeois C et al. PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments. Nat Neurosci 18:36-45 (2015). PubMed: 25485759
