Product nameAnti-Plexin A1 antibody [EPR3449]
See all Plexin A1 primary antibodies
DescriptionRabbit monoclonal [EPR3449] to Plexin A1
Specificityab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Plexin A1 aa 100-200. The exact sequence is proprietary.
- WB: KS62, HUVEC and HepG2 whole cell lysate (ab7900); fetal brain tissue lysate FC: K562 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesThis antibody is not purified. It is provided in cell supernatant and storage buffer.
Our Abpromise guarantee covers the use of ab92346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 211 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceFunction: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Tissue specificity: Detected in fetal brain, lung, liver and kidney. Similarity: Belongs to the plexin family. Contains 4 IPT/TIG domains. Contains 1 Sema domain.
Cellular localizationCell Membrane
- NOV antibody
- NOV/plexin A1 protein antibody
- NOVP antibody
All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 211 kDa
Flow cytometry analysis of permeabilized K562 cells labeling Plexin A1 with ab92346 at 1/50 (red) campared to a rabbit IgG (negative control) (green).