Product nameAnti-Plexin A2 antibody
See all Plexin A2 primary antibodies
DescriptionRabbit polyclonal to Plexin A2
Tested applicationsSuitable for: WB, IHC-P, IHC-FoFrmore details
Species reactivityReacts with: Mouse
Predicted to work with: HumanDoes not react with: Rat
- This antibody gave a positive signal in Mouse Brain (0 Day) Tissue Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).|
FunctionCoreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
Tissue specificityDetected in fetal brain.
Sequence similaritiesBelongs to the plexin family.
Contains 4 IPT/TIG domains.
Contains 1 Sema domain.
- Information by UniProt
- Plexin-A2 antibody
- PlexinA2 antibody
- PLXA2 antibody
IHC-FoFr image of Plexin A2 staining on mouse cortex (Lamina V) using ab39357 (1/300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Anti-Plexin A2 antibody (ab39357) at 2 µg/ml +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 212 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunofluorescence analysis of mouse neural tube cells, staining Plexin A2 (green) with ab39357.
Cells were fixed before being blocked with 10% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
This product has been referenced in:
- Sabag AD et al. The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction. J Cell Sci 127:5240-52 (2014). Read more (PubMed: 25335892) »
- Li W et al. Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. Proc Natl Acad Sci U S A 110:1738-43 (2013). Read more (PubMed: 23319608) »