Anti-Plexin A2 antibody (ab39357)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-Plexin A2 antibody
See all Plexin A2 primary antibodies -
DescriptionRabbit polyclonal to Plexin A2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IHC-FoFrmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Human
Does not react with:
Rat -
Immunogen
Synthetic peptide derived from within residues 300 - 400 of Mouse Plexin A2.
Read Abcam's proprietary immunogen policy (Peptide available as ab39356.) -
Positive control
- This antibody gave a positive signal in Mouse Brain (0 Day) Tissue Lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab39357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa). | |
| IHC-P | 1/100. | |
| IHC-FoFr | 1/300. |
Target
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FunctionCoreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
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Tissue specificityDetected in fetal brain.
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Sequence similaritiesBelongs to the plexin family.
Contains 4 IPT/TIG domains.
Contains 1 Sema domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 5362 Human
- Entrez Gene: 18845 Mouse
- Omim: 601054 Human
- SwissProt: O75051 Human
- SwissProt: P70207 Mouse
- Unigene: 497626 Human
- Unigene: 2251 Mouse
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Alternative names
- Plexin-A2 antibody
- PlexinA2 antibody
- PLXA2 antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin A2 antibody (ab39357)This image is courtesy of an abreview submitted by Sophie Pezet, ESPCI, FranceIHC-FoFr image of Plexin A2 staining on mouse cortex (Lamina V) using ab39357 (1/300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique. -
Western blot - Anti-Plexin A2 antibody (ab39357)Anti-Plexin A2 antibody (ab39357) at 2 µg/ml +Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
Secondary
Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 212 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunocytochemistry/ Immunofluorescence - Anti-Plexin A2 antibody (ab39357)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of mouse neural tube cells, staining Plexin A2 (green) with ab39357.
Cells were fixed before being blocked with 10% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
Datasheets and documents
References
This product has been referenced in:
- Sabag AD et al. The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction. J Cell Sci 127:5240-52 (2014). Read more (PubMed: 25335892) »
- Li W et al. Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. Proc Natl Acad Sci U S A 110:1738-43 (2013). Read more (PubMed: 23319608) »
