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  1. Link

    plexin-a2-antibody-ab39357.pdf

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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins
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Anti-Plexin A2 antibody (ab39357)

  • Datasheet
Reviews (3) Submit a question References (5)

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Western blot - Anti-Plexin A2 antibody (ab39357)
  • Immunocytochemistry/ Immunofluorescence - Anti-Plexin A2 antibody (ab39357)

Key features and details

  • Rabbit polyclonal to Plexin A2
  • Suitable for: WB, IHC-P, IHC-FoFr
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-Plexin A2 antibody
  • Description

    Rabbit polyclonal to Plexin A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: HumanDoes not react with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Plexin A2 aa 300-400.
    (Peptide available as ab39356)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).
IHC-P
Use at an assay dependent concentration.
IHC-FoFr (1)
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 212 kDa).
IHC-P
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.

Target

  • Function

    Coreceptor for SEMA3A. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
  • Tissue specificity

    Detected in fetal brain.
  • Sequence similarities

    Belongs to the plexin family.
    Contains 4 IPT/TIG domains.
    Contains 1 Sema domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession O75051 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5362 Human
    • Entrez Gene: 18845 Mouse
    • Omim: 601054 Human
    • SwissProt: O75051 Human
    • SwissProt: P70207 Mouse
    • Unigene: 497626 Human
    • Unigene: 2251 Mouse
    • Alternative names

      • Plexin-A2 antibody
      • PlexinA2 antibody
      • PLXA2 antibody
      • PLXA2_HUMAN antibody
      • PLXNA2 antibody
      • Semaphorin receptor OCT antibody
      see all

    Images

    • Western blot - Anti-Plexin A2 antibody (ab39357)
      Western blot - Anti-Plexin A2 antibody (ab39357)
      Anti-Plexin A2 antibody (ab39357) at 2 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

      Secondary
      Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 212 kDa
      Observed band size: 230 kDa why is the actual band size different from the predicted?



      Plexin A2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    • Immunocytochemistry/ Immunofluorescence - Anti-Plexin A2 antibody (ab39357)
      Immunocytochemistry/ Immunofluorescence - Anti-Plexin A2 antibody (ab39357)This image is courtesy of an anonymous Abreview
      Immunofluorescence analysis of mouse neural tube cells, staining Plexin A2 (green) with ab39357.

      Cells were fixed before being blocked with 10% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/100 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

      See Abreview

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (5)

    Publishing research using ab39357? Please let us know so that we can cite the reference in this datasheet.

    ab39357 has been referenced in 5 publications.

    • Bi-Lin KW  et al. Critical role of the BAF chromatin remodeling complex during murine neural crest development. PLoS Genet 17:e1009446 (2021). PubMed: 33750945
    • Smolkin T  et al. Complexes of plexin-A4 and plexin-D1 convey semaphorin-3C signals to induce cytoskeletal collapse in the absence of neuropilins. J Cell Sci 131:N/A (2018). PubMed: 29661844
    • Sabag AD  et al. The role of the plexin-A2 receptor in Sema3A and Sema3B signal transduction. J Cell Sci 127:5240-52 (2014). PubMed: 25335892
    • Li W  et al. Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. Proc Natl Acad Sci U S A 110:1738-43 (2013). PubMed: 23319608
    • Piaton G  et al. Class 3 semaphorins influence oligodendrocyte precursor recruitment and remyelination in adult central nervous system. Brain 134:1156-67 (2011). PubMed: 21421691

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Plexin A2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Neural Tube)
    Specification
    Neural Tube
    Fixative
    IF-P
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2012

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Plexin A2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    DR. Sophie Pezet

    Verified customer

    Submitted Feb 08 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Plexin A2 antibody

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (embryo d15 spinal cord, kidney, adult brain)
    Specification
    embryo d15 spinal cord, kidney, adult brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 22 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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