• Product name

  • Description

    Rabbit polyclonal to Plexin A4
  • Host species

  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IF, IHC-FrFlmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Plexin A4 aa 500-600 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab39349)

  • Positive control

    • ab39350 gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Mouse Brain (P0); Mouse Dorsal Root Ganglia.



Our Abpromise guarantee covers the use of ab39350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 213 kDa (predicted molecular weight: 213 kDa).Can be blocked with Mouse Plexin A4 peptide (ab39349).
ICC/IF Use a concentration of 1 µg/ml.
IHC-FrFl Use at an assay dependent concentration.


  • Function

    Forms a receptor complex with neuropilin-1 and can propagate semaphorin-3A elicited inhibitory signals into cells and neurons.
  • Sequence similarities

    Belongs to the plexin family.
    Contains 4 IPT/TIG domains.
    Contains 3 PSI domains.
    Contains 1 Sema domain.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • FAYV2820 antibody
    • KIAA1550 antibody
    • PLEXA4 antibody
    • Plexin-A4 antibody
    • PlexinA4 antibody
    • PLXA4_HUMAN antibody
    • plxna4 antibody
    • PLXNA4A antibody
    • PLXNA4B antibody
    • PRO34003 antibody
    • UNQ2820/PRO34003 antibody
    see all


  • ICC/IF image of ab39350 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39350, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-Plexin A4 antibody (ab39350) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/35000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 213 kDa
    Observed band size: 213 kDa
    Additional bands at: 190 kDa, 41 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab39350 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-Plexin A4 antibody (ab39350) at 1/250 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 3 : Dorsal Root Ganglia (Mouse) Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
    Lane 5 : Mouse brain tissue lysate - total protein (0 days) (ab7188) with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
    Lane 6 : Dorsal Root Ganglia (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Lanes 1-2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
    Lanes 3-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 213 kDa

    We are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.

  • ab39350 staining Plexin A4 in mouse brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

    See Abreview


This product has been referenced in:

  • Lee FH  et al. Disrupting GluA2-GAPDH Interaction Affects Axon and Dendrite Development. Sci Rep 6:30458 (2016). IHC-P ; Mouse . Read more (PubMed: 27461448) »
  • Yamashita N  et al. Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors. Nat Commun 5:3424 (2014). Read more (PubMed: 24599038) »
See all 7 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Tris pH9.0 + 1mM EDTA

Dr. Yves Heremans

Verified customer

Submitted Jun 30 2017


Thank you for your reply.

I am sorry to hear that the replacement antibody didn't work for you. This is the only lot in stock and we do not plan on making a new batch until we sell the 15 vials we have in stock.

I would be happy to refund your original order since the replacement didn't work. Please let me know if this is how you would like to proceed.

I look forward to your reply.

Read More


Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More


Thank you for contacting us.

Anja is on her well deserved holiday, therefore, I will deal with your case.

It is very possible that Gutekunstet al. used another lot: so far, we have produced more than 50 lots of this antibody. We take good care that we sell only lots, which have passed our quality control. Therefore, we are very keen to find out why the antibody didn't work in your hands.

I hope this information is helpful to you. We are looking forward to receive the questionnaire to see if we could help you in this case.

Use our products? Submit an Abreview. Earn rewards!

Read More
Immunohistochemistry free floating
Mouse Tissue sections (Brain)

Abcam user community

Verified customer

Submitted Aug 18 2009

Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain sections)
Brain sections
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Mar 17 2009

Immunocytochemistry/ Immunofluorescence
Rat Cultured Cells (dissociated cortical neurons)
dissociated cortical neurons
Yes - 0.1% triton X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Dr. Ioana Carcea

Verified customer

Submitted Apr 08 2008

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