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Immunohistochemistry - Free Floating abreview for Anti-Plexin A4 antibody

Good
Abreviews
Go to datasheet (ab39350)
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 1% serum + 0.2% Triton

Secondary antibody

Name
Abcam antibody:Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin)
Dilution
1/200

Additional data

Additional Notes
Antigen retrieval - citric acid pH 4.5 at 95C for 5min - had to be used to obtain good staining. Antibody labeled distinct cell populations and fibers very clearly with low background as seen in the image below (20x magnification).

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Submitted Aug 18 2009