Anti-Plexin B1 antibody (ab39717)
Key features and details
- Rabbit polyclonal to Plexin B1
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-Plexin B1 antibody
See all Plexin B1 primary antibodies -
Description
Rabbit polyclonal to Plexin B1 -
Host species
Rabbit -
Specificity
PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved. -
Tested applications
Suitable for: IHC-FoFr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.
Read Abcam's proprietary immunogen policy (Peptide available as ab41062.) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab39717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa). |
Target
-
Relevance
Plexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton. -
Cellular localization
Secreted and single pass type I membrane protein. -
Database links
- Entrez Gene: 5364 Human
- Entrez Gene: 235611 Mouse
- Entrez Gene: 316009 Rat
- Omim: 601053 Human
- SwissProt: O43157 Human
- SwissProt: Q8CJH3 Mouse
-
Alternative names
- KIAA0407 antibody
- Plexin 5 antibody
- Plexin B1 antibody
see all
Images
-
Western blot - Anti-Plexin B1 antibody (ab39717)All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 233 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 155 kDa. We are unsure as to the identity of these extra bands.
We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).
Protocols
Datasheets and documents
References (1)
ab39717 has been referenced in 1 publication.
- Daviaud N et al. Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol 76:882-99 (2016). PubMed: 26579598
