Anti-Plexin B1 antibody (ab39717)

Reviews (2)Q&A (1)References (1)

Overview

  • Product name
    Anti-Plexin B1 antibody
    See all Plexin B1 primary antibodies
  • Description
    Rabbit polyclonal to Plexin B1
  • Host species
    Rabbit
  • Specificity
    PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved.
  • Tested applications
    Suitable for: IHC-FoFr, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41062.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver Rat Liver Rat Pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab39717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).

Target

  • Relevance
    Plexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton.
  • Cellular localization
    Secreted and single pass type I membrane protein.
  • Database links
    • Alternative names
      • KIAA0407 antibody
      • Plexin 5 antibody
      • Plexin B1 antibody
      • PLXN5 antibody
      • PLXNB1 antibody
      • Semaphorin receptor antibody
      • Semaphorin receptor SEP antibody
      • SEP antibody
      see all

    Images

    • Western blot - Anti-Plexin B1 antibody (ab39717)
      Western blot - Anti-Plexin B1 antibody (ab39717)
      All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Liver (Rat) Tissue Lysate
      Lane 3 : Pancreas (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 233 kDa
      Observed band size: 171 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 155 kDa. We are unsure as to the identity of these extra bands.



      We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1.
    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)This image is courtesy of Sophie Pezet, CNRS, Paris, France

      Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).

    References

    This product has been referenced in:
    See 1 Publication for this product

    Publishing research using ab39717? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Plexin B1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cultured Cells (Primary dissection of hippocampal neurons.)
    Specification
    Primary dissection of hippocampal neurons.
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Dr. Michael Weible

    Verified customer

    Submitted Nov 08 2012

    Question

    Please see a request we have received from a customer


    Thank you for your email. Yes, I would be happy to test the antibodies and share the data with Abcam.


    If you could request a code for the following antibodies that would be great:



    1. Products catalogue number: Anti-Plexin B1 antibody (ab39717)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: neurons cultured from dorsal root ganglia, trigeminal ganglia and hippocampus.


    1. Products catalogue number: Anti-Dlx2 antibody [4B9] (ab117546)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: Adult neural stem/progenitor cells derived from the SVZ and cultured as neurospheres.

    1. Products catalogue number: Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: Adult neural stem/progenitor cells derived from the SVZ and cultured as neurospheres.

    1. Products catalogue number: Anti-SOX2 antibody (ab97959)
    2. Which application the customer would like to test: Immunocytochemistry (ICC)
    3. Which species: Mouse C57
    4. Their sample type: Adult neural stem/progenitor cells derived from the SVZ and cultured as neurospheres.

    Read More
    Answer



    Expiration date: 2012-11-09


    I am very pleased to hear you would like to accept our offer and test

    ab38556 Anti-MASH1/Achaete-scute homolog 1 antibody
    ab39717 Anti-Plexin B1 antibody
    ab117546 Anti-Dlx2 antibody [4B9]



    in ICC. One code will give you: 1 free primary before the expiration date. To redeem this offer, please submit an Abreview for ICCand include this code in the "Additional Comments" section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.


    In addition, ab97959 Anti-SOX2 antibodyhas already been tested for mouse as well as for ICC. Therefore, itis not a testing-discount candidate.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Plexin B1 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Feb 24 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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