• Product name

  • Description

    Rabbit polyclonal to Plexin B1
  • Host species

  • Specificity

    PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved.
  • Tested applications

    Suitable for: IHC-FoFr, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41062.)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver Rat Liver Rat Pancreas



Our Abpromise guarantee covers the use of ab39717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).


  • Relevance

    Plexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton.
  • Cellular localization

    Secreted and single pass type I membrane protein.
  • Database links

  • Alternative names

    • KIAA0407 antibody
    • Plexin 5 antibody
    • Plexin B1 antibody
    • PLXN5 antibody
    • PLXNB1 antibody
    • Semaphorin receptor antibody
    • Semaphorin receptor SEP antibody
    • SEP antibody
    see all


  • All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 233 kDa
    Observed band size: 171 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 155 kDa. We are unsure as to the identity of these extra bands.

    We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1.
  • Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).


This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Mouse Cultured Cells (Primary dissection of hippocampal neurons.)
Primary dissection of hippocampal neurons.
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Michael Weible

Verified customer

Submitted Nov 08 2012


Expiration date: 2012-11-09

I am very pleased to hear you would like to accept our offer and test

ab38556 Anti-MASH1/Achaete-scute homolog 1 antibody
ab39717 Anti-Plexin B1 antibody
ab117546 Anti-Dlx2 antibody [4B9]

in ICC. One code will give you: 1 free primary before the expiration date. To redeem this offer, please submit an Abreview for ICCand include this code in the "Additional Comments" section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

In addition, ab97959 Anti-SOX2 antibodyhas already been tested for mouse as well as for ICC. Therefore, itis not a testing-discount candidate.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (Brain sections)
Brain sections
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Feb 24 2010

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