Anti-Plexin B1 antibody (ab39717)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Plexin B1 antibody
See all Plexin B1 primary antibodies -
DescriptionRabbit polyclonal to Plexin B1
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Host speciesRabbit
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SpecificityPLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved.
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Tested applicationsSuitable for: IHC-FoFr, ICC, WBmore details
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Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.
Read Abcam's proprietary immunogen policy (Peptide available as ab41062.) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Liver Rat Liver Rat Pancreas
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab39717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. | |
| ICC | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa). |
Target
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RelevancePlexin B1 is the transmembrane receptor for the class 4 semaphorin, SEMA4D. Plexin B1 activation plays a role in axon guidance, invasive growth and cell migration and has been reported to have additional roles in RHOA activation and subsequent changes of the actin cytoskeleton.
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Cellular localizationSecreted and single pass type I membrane protein.
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Database links
- Entrez Gene: 5364 Human
- Entrez Gene: 235611 Mouse
- Entrez Gene: 316009 Rat
- Omim: 601053 Human
- SwissProt: O43157 Human
- SwissProt: Q8CJH3 Mouse
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Alternative names
- KIAA0407 antibody
- Plexin 5 antibody
- Plexin B1 antibody
see all
Images
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Western blot - Anti-Plexin B1 antibody (ab39717)All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 233 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 155 kDa. We are unsure as to the identity of these extra bands.
We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Daviaud N et al. Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol 76:882-99 (2016). Read more (PubMed: 26579598) »
