Anti-Plexin B2/MM1 antibody (ab229950)
Key features and details
- Rabbit polyclonal to Plexin B2/MM1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Plexin B2/MM1 antibody
See all Plexin B2/MM1 primary antibodies -
Description
Rabbit polyclonal to Plexin B2/MM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Plexin B2/MM1 aa 1050-1200.
Database link: O15031 -
Positive control
- WB: Mouse liver lysate. IHC-P: Human colon cancer and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 205 kDa (predicted molecular weight: 205 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Relevance
Plexin B2/MM1 is a transmembrane receptor for the class 4 semaphorin, SEMA4D. This receptor has key roles in RHOA activation and subsequent changes of the actin cytoskeleton, axon guidance, invasive growth and cell migration. -
Cellular localization
Membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 23654 Human
- Entrez Gene: 140570 Mouse
- Omim: 604293 Human
- SwissProt: O15031 Human
- SwissProt: Q3UH76 Mouse
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Alternative names
- KIAA0315 antibody
- MM1 antibody
- Plexin B2 antibody
see all
Images
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Anti-Plexin B2/MM1 antibody (ab229950) at 1/1000 dilution + Mouse liver lysate
Secondary
Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 205 kDa
Observed band size: 205 kDa -
Paraffin-embedded human colon cancer tissue stained for Plexin B2/MM1 using ab229950 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for Plexin B2/MM1 using ab229950 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229950 has been referenced in 1 publication.
- Lin L et al. A circular RNA derived from PLXNB2 as a valuable predictor of the prognosis of patients with acute myeloid leukaemia. J Transl Med 19:123 (2021). PubMed: 33757550