Overview

  • Product name
    Anti-PLGF antibody [37203.111]
    See all PLGF primary antibodies
  • Description
    Mouse monoclonal [37203.111] to PLGF
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human), expressed in Escherichia coli.

  • Positive control
    • Human umbilical vein endothelial cells.
  • General notes
    Do not store in frost-free freezer. Endotoxin level is <10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: For capture ELISAs, this product can be used as the capture antibody. Use at a working concentration of 2 µg/ml (100 µl/well) in combination with a biotinylated PlGF polyclonal detection antibody at 100 ng/ml (100 µl/well).
    WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. Homodimeric recombinant PlGF migrates as an approximately 29 kDa protein.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
    • Tissue specificity
      While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
    • Sequence similarities
      Belongs to the PDGF/VEGF growth factor family.
    • Domain
      Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
    • Information by UniProt
    • Database links
    • Alternative names
      • D12S1900 antibody
      • Pgf antibody
      • PGFL antibody
      • PIGF antibody
      • Placenta growth factor antibody
      • Placental growth factor antibody
      • Placental growth factor, vascular endothelial growth factor related protein antibody
      • PlGF 2 antibody
      • PlGF antibody
      • PLGF_HUMAN antibody
      • PlGF2 antibody
      • SHGC 10760 antibody
      see all

    References

    ab10966 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Answer

    Thank you for getting back to me and I'm happy to hear that ab10966 worked well for you. We would be very grateful for you if you could submit an Abreview via the product datasheet. We always encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. We hope to receive your Abreview very shortly. Thank you for your time and effort.

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    Answer

    Thank you for your email. I have arranged for a free of charge replacement vial of ab10966 to be sent to you on order# 77020. Lot 95559 is the only batch that we currently have in stock. It is a possibility that your previous vial had gone off during the shipping process. Please let me know how this replacement vial works out for you and if you have any additional questions.

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    Answer

    Thank you for your email. At this point I would like to offer you a replacement vial free of charge or a refund, please let me know which you would prefer. Thanks again, and I look forward to hearing from you.

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    Answer

    Thank you for your email. I'm replying to with regard to this issue as my colleague Tanya is away for the Easter holidays. With the HUVEC lysate, what dilutions of ab10966 did you try? Did you incubate overnight at 4C? I apologize if this information was in your previous email, the bottom may have been accidently deleted. Thanks again, and have a great weekend.

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    Answer

    Thank you for contacting us and for providing details of your Western blot protocol. We are very sorry to hear that you are having problem with this antibody. We have read through your message and would like to make the following suggestions/comments: 1. Although we suggest using this antibody at a concentration of 1 - 2 µg/ml, this range is just a recommendation. This product was tested and characterised using recombinant protein. The detection limit for recombinant human placental growth factor is approximately 20 ng/lane and 300 ng/lane under non-reducing and reducing conditions, respectively. We would strongly suggest running purified recombinant protein as positive control along with your samples. 2. If this target protein is expressed at lower levels, then you need to optimize the concentration. Do you know the expressed levels of the Placental Growth Factor in the keratinocytes? If you can't have recombinant protein, then you need to use Human umbilical vein endothelial cells as positive control. 3. You have not mentioned in your e-mail from which species the cells come from? Are they human, mouse, rat etc? This antibody recognizes only human Placental Growth Factor, we have not tested the cross-reactivity with other species. 4. Always use freshly made lysis buffer with a cocktail of proteinase inhibitors. We normally suggest using RIPA buffer. 5. Incubate the membrane with the primary antibody overnight at 4oC to see if the signal is getting stronger or not. 6. Have you tested the transfer of the proteins with Ponceau or other type of dye? How do the molecular weight markers look like? 7. Please also test the secondary antibody with another primary antibody. 8. Finally, we would suggest testing whole cell lysate (make samples before the centrifugation step in case the target you may lose some material in the pellet). Load 20-30 ug per ml of total protein per lane but not more than that other wise the gel may be overloaded. We hope this information will be useful for you. Please find enclose Preparation of RIPA cell lysates for Western Blots: 1. Wash cell pellets once with ice-cold PBS. 2. Add 1 ml of RIPA buffer to 10x8 cells, incubate on ice for 20 min, vortex 2 to 3 times. 3. Centrifuge for 5 min at 4oC at maximum speed in a microfuge tube. 4. Transfer supernatant into clean tube. Measure protein concentration with a protein assay. 5. Adjust concentration to 5 mg/ml with RIPA lysis buffer. 6. Add equal volume of 2 x SDS sample buffer into cell lysate, boil for 5 min. 7. Store at -20oC for daily use or -80oC for long term. Avoid repeated freeze thaw cycles. RIPA Base Ingredients Tris-HCl: 50 mM, pH 7.4 NP-40: 1% Na-deoxycholate: 0.25% NaCl: 150 mM EDTA: 1 mM PMSF: 1 mM Aprotinin, leupeptin, pepstatin: 1 microgram/ml each RIPA Protease Inhibitors Phenylmethylsulfonyl fluoride (PMSF) (200 mM stock solution in isopropanol; store at room temperature) EDTA (calcium chelator; 100 mM stock solution in H2O, pH 7.4) Leupeptin (store frozen in aliquots, 1 mg/ml in H2O) Aprotinin (store frozen in aliquots, 1 mg/ml in H2O) Pepstatin (store frozen in aliquots, 1 mg/ml in methanol) Good luck! Should you need further assistance, then please do not hesitate to contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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