Overview

  • Product name
  • Description
    Rabbit polyclonal to PLGF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PLGF (N terminal).
    Database link: P49763-3

  • Positive control
    • BT474 cell extract; Mouse brain tissue extract; Rat testis, Human stomach cancer and Rat kidney tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab196666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.

Target

  • Function
    Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
  • Tissue specificity
    While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Domain
    Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
  • Information by UniProt
  • Database links
  • Alternative names
    • D12S1900 antibody
    • Pgf antibody
    • PGFL antibody
    • PIGF antibody
    • Placenta growth factor antibody
    • Placental growth factor antibody
    • Placental growth factor, vascular endothelial growth factor related protein antibody
    • PlGF 2 antibody
    • PlGF antibody
    • PLGF_HUMAN antibody
    • PlGF2 antibody
    • SHGC 10760 antibody
    see all

Images

  • All lanes : Anti-PLGF antibody (ab196666) at 1/500 dilution

    Lane 1 : BT474 cell extract
    Lane 2 : Mouse brain tissue extract

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of paraffin embedded Rat testis tissue labeling PLGF with ab196666 at 1/200 dilution. (400x magnification).

  • Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue labeling PLGF with ab196666 at 1/200 dilution.  (400x magnification).

  • Immunohistochemical analysis of paraffin embedded Rat kidney tissue labeling PLGF with ab196666 at 1/200 dilution.  (400x magnification).

References

This product has been referenced in:
  • Gimble JM  et al. A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model. Plast Reconstr Surg Glob Open 6:e2010 (2018). Read more (PubMed: 30881803) »
  • Naik A  et al. Neuropilin-1 Associated Molecules in the Blood Distinguish Poor Prognosis Breast Cancer: A Cross-Sectional Study. Sci Rep 7:3301 (2017). IHC-P ; Human . Read more (PubMed: 28607365) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196666.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up