Product nameAnti-PLGF antibody
See all PLGF primary antibodies
DescriptionRabbit polyclonal to PLGF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Pig
Synthetic peptide corresponding to Human PLGF aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human PLGF1 protein (ab50127) can be used as a positive control in WB. This antibody gave a positive signal in Human Placenta Tissue Lysate, and JEG-3, SW480 and RAW 264.7 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab83906 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 26 kDa).|
FunctionGrowth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
Tissue specificityWhile the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
DomainIsoform PlGF-2 contains a basic insert which acts as a cell retention signal.
Cellular localizationSecreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
- Information by UniProt
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
All lanes : Anti-PLGF antibody (ab83906) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Ab83906 detects a band of approximately 55-kDa. While this differs to its predicted molecular weight of 26-kDa, this migration has been observed in the literature (PMID: 10403932).
ab83906 has not yet been referenced specifically in any publications.