Key features and details
- Rabbit polyclonal to PLGF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLGF antibody
See all PLGF primary antibodies
DescriptionRabbit polyclonal to PLGF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Pig
Synthetic peptide corresponding to Human PLGF aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human PLGF1 protein (ab50127) can be used as a positive control in WB. This antibody gave a positive signal in Human Placenta Tissue Lysate, and JEG-3, SW480 and RAW 264.7 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab83906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 26 kDa).|
FunctionGrowth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
Tissue specificityWhile the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
DomainIsoform PlGF-2 contains a basic insert which acts as a cell retention signal.
Cellular localizationSecreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
- Information by UniProt
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
All lanes : Anti-PLGF antibody (ab83906) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Ab83906 detects a band of approximately 55-kDa. While this differs to its predicted molecular weight of 26-kDa, this migration has been observed in the literature (PMID: 10403932).
ab83906 has not yet been referenced specifically in any publications.