• Product name
    Anti-PLGF antibody - N-terminal
    See all PLGF primary antibodies
  • Description
    Rabbit polyclonal to PLGF - N-terminal
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
    Database link: P49763

  • Positive control
    • WB: Purified, recombinant PLGF-1/-2. ICC/IF: HepG2 cells.
  • General notes

    According to Swissprot P49763; PLGF exists in three isoforms PLGF 1, 2 and 3. The N-terminal immunogen sequence of ab9542 is present in all isoforms so extra bands might be observed near the band of interest.



Our Abpromise guarantee covers the use of ab9542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 5 µg/ml.

Allows the detection of 1.0 - 2.5 ng/well of recombinant human PLGF-1 and PLGF-2.

WB Use a concentration of 1 - 4 µg/ml.

It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane.

IHC-P Use at an assay dependent concentration. PubMed: 19336420
ICC/IF Use a concentration of 1 µg/ml.


  • Function
    Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
  • Tissue specificity
    While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Domain
    Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.
  • Post-translational
  • Cellular localization
    Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
  • Information by UniProt
  • Database links
  • Alternative names
    • D12S1900 antibody
    • Pgf antibody
    • PGFL antibody
    • PIGF antibody
    • Placenta growth factor antibody
    • Placental growth factor antibody
    • Placental growth factor, vascular endothelial growth factor related protein antibody
    • PlGF 2 antibody
    • PlGF antibody
    • PLGF_HUMAN antibody
    • PlGF2 antibody
    • SHGC 10760 antibody
    see all


  • Western blot of chromatography purified, recombinant PLGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
    PlGF antibody shows no cross-reactivity to VEGF165.

  • ICC/IF image of ab9542 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9542, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.


This product has been referenced in:
  • Beiswenger TR  et al. The effect of cigarette smoke extract on trophoblast cell viability and migration: the role of adrenomedullin. Reprod Sci 19:526-33 (2012). WB ; Human . Read more (PubMed: 22267538) »
  • Mansfield AS  et al. Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis. Mod Pathol 24:487-94 (2011). IHC-P ; Human . Read more (PubMed: 21151098) »

See all 12 Publications for this product

Customer reviews and Q&As

Your credit note ID is XXXXX. Please pass this number to your finance department and ask them to quote this number when ordering new products. Once order received we will automatically deduct the money from invoice.

I hope this information wil...

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Thank you for contacting us.

Your credit note ID is 23009.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these PLGF antibodies.

In answer to your question, I would like to reassure you that as far as I can see from our rec...

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Thank you for your reply.

That would not be a problem. However, could you please provide me with the order number on which ab9542 was received?

Many thanks in advance for your cooperation in this case.

Thank you for contacting us. xxxx is currently away form the office but I will try to help as best I can.

I am sorry to hear that the protocol tips provided have not resulted in an improvement of the Western blotting performed. I the customer...

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Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been successful. In order to help with our investigation of this case before deciding how to...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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You email has been forward to me by my colleague.

The IHC-P dilution, 1/1000 is for guidelines purpose only; you can use this dilution as a start however if you observe faint staining then you might need more concentrated antibody e.g. 1/500 d...

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Thank you for your email.

- No primary control, is a negative control which is used to check the non specific binding of secondry antibody.

Please check my emails in comparison to the literature. http://www.uniprot.org/uniprot/P4976...

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Thank you for your email.

The only reason for double band is dimer of PLGF protein. Please re do the WB with following suggestions;

- Heat lysates with sample buffer 10 minutes at 100C.
- Load 30ug protein per well
- Try n...

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1-10 of 22 Abreviews or Q&A


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