Anti-PLGF antibody - N-terminal (ab9542)
Key features and details
- Rabbit polyclonal to PLGF - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-PLGF antibody - N-terminal
See all PLGF primary antibodies -
Description
Rabbit polyclonal to PLGF - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human PLGF (N terminal). Highly purified N-terminal 20 aa synthetic peptide (Human).
Database link: P49763 -
General notes
According to Swissprot P49763; PLGF exists in three isoforms PLGF 1, 2 and 3. The N-terminal immunogen sequence of ab9542 is present in all isoforms so extra bands might be observed near the band of interest.
Properties
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Form
Lyophilized:Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Recombinant fragment
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 4 µg/ml.
It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane. |
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 4 µg/ml. It will detect 25 - 50 ng/lane of recombinant human PLGF-1 (17-18 kDa) and PLGF-2 (22-23 kDa) under reducing conditions. Dimers are detected at higher protein amounts/lane. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
Tissue specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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Database links
- Entrez Gene: 5228 Human
- Omim: 601121 Human
- SwissProt: P49763 Human
- Unigene: 252820 Human
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Alternative names
- D12S1900 antibody
- Pgf antibody
- PGFL antibody
see all
Images
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Western blot - Anti-PLGF antibody - N-terminal (ab9542)
Western blot of chromatography purified, recombinant PLGF-1/-2 and VEGF165 immobilized to PVDF-membrane.
PlGF antibody shows no cross-reactivity to VEGF165. -
Immunocytochemistry/ Immunofluorescence - Anti-PLGF antibody - N-terminal (ab9542)ICC/IF image of ab9542 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9542, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Protocols
Datasheets and documents
References (17)
ab9542 has been referenced in 17 publications.
- Balser C et al. Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retina. J Neuroinflammation 16:26 (2019). PubMed: 30732627
- Jiao W et al. Distinct downstream signaling and the roles of VEGF and PlGF in high glucose-mediated injuries of human retinal endothelial cells in culture. Sci Rep 9:15339 (2019). PubMed: 31653890
- Meng Q et al. Expression of Placenta Growth Factor Is Associated with Unfavorable Prognosis of Advanced-Stage Serous Ovarian Cancer. Tohoku J Exp Med 244:291-296 (2018). PubMed: 29643276
- Felker AM & Croy BA Natural cytotoxicity receptor 1 in mouse uNK cell maturation and function. Mucosal Immunol 10:1122-1132 (2017). PubMed: 28098245
- Chen X et al. Differential expression of vascular endothelial growth factor angiogenic factors in different endometrial compartments in women who have an elevated progesterone level before oocyte retrieval, during in vitro fertilization-embryo transfer treatment. Fertil Steril 104:1030-1036 (2015). PubMed: 26143364
