Anti-PLK1 antibody [35-206] (ab17056)

Mouse monoclonal PLK1 antibody [35-206]. Validated in WB, IP, IHC, ICC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 21 publication(s). Independently reviewed in 5 review(s).


  • Product name
    Anti-PLK1 antibody [35-206]
    See all PLK1 primary antibodies
  • Description
    Mouse monoclonal [35-206] to PLK1
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-P, IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    His-PLK1 full length purified from Sf9 cells.

  • Epitope
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab17056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 67 kDa). This clone is superior to clone 36-298 (ab17057) in Western blotting and IP, but suffers from high background in IF and ICC.
ICC Use at an assay dependent concentration.


  • Function
    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
  • Tissue specificity
    Placenta and colon.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.
  • Developmental stage
    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
  • Post-translational
    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle regulated protein kinase antibody
    • PLK 1 antibody
    • PLK antibody
    • PLK-1 antibody
    • plk1 antibody
    • PLK1_HUMAN antibody
    • Polo like kinase 1 antibody
    • Polo-like kinase 1 antibody
    • Serine/threonine protein kinase 13 antibody
    • Serine/threonine protein kinase PLK1 antibody
    • Serine/threonine-protein kinase 13 antibody
    • Serine/threonine-protein kinase PLK1 antibody
    • STPK 13 antibody
    • STPK13 antibody
    see all


  • Anti-PLK1 antibody [35-206] (ab17056) at 1 µg/ml + A431 cell lysate at 20 µg

    Rabbit-anti mouse Alexa fluor(680) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • Immunofluoresence using ab17056 and either U2OS, HeLaS3 or NIH 3T3 cells.

    Plk1 can be seen localising with MKLP in the U2OS cell and with Cdc20 in the HeLaS3 and NIH 3T3 cells shown.

  • All lanes : Anti-PLK1 antibody [35-206] (ab17056)

    Lane 1 : Recombinant PLK1
    Lane 2 : U2OS lysate
    Lane 3 : HeLaS3

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 66 kDa why is the actual band size different from the predicted?

    Western blot using ab17056.

    Lane 1: recombinant PLK1
    Lane 2: U2OS lysate
    Lane 3: HeLaS3 lysate

    15% SDS-PAGE gel

  • ab17056 at 1/100 staining human kidney tubular epithelial cells by ICC/IF. The cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked with goat serum before incuabtion with the antibody. A goat anti-mouse FITC antibody was used as the secondary.

    See Abreview

  • Overlay histogram showing HCT116 cells stained with ab17056 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17056, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Li D  et al. Synergistically enhanced anticancer effect of codelivered curcumin and siPlk1 by stimuli-responsive a-lactalbumin nanospheres. Nanomedicine (Lond) 14:595-612 (2019). Read more (PubMed: 30806584) »
  • Miyazaki S  et al. Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I. Genes Cells 22:552-567 (2017). IF . Read more (PubMed: 28497540) »
See all 22 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Immunocytochemistry/ Immunofluorescence
Pig Cell (kidney epithelial)
Yes - tritonX100
kidney epithelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Patricia Wadsworth

Verified customer

Submitted Jan 31 2017

Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12% MOPS)
Human Cell lysate - whole cell (HEK293)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 29 2014

Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Human Cell lysate - whole cell (Colon carcinoma)
Colon carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Eleni Petsalaki

Verified customer

Submitted Dec 04 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer
Yes - Buffer from Dako with Tween20

Mr. Rudolf Jung

Verified customer

Submitted May 06 2011

Immunocytochemistry/ Immunofluorescence
Human Cell (kidney tubular epithelium)
kidney tubular epithelium
Yes - Triton X-100
Blocking step
Goat serum (10% in PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Melanie Adler

Verified customer

Submitted Aug 03 2007

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