Key features and details
- Mouse monoclonal [36-298] to PLK1 - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt, Indirect ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-PLK1 antibody [36-298] - BSA and Azide free
See all PLK1 primary antibodies
DescriptionMouse monoclonal [36-298] to PLK1 - BSA and Azide free
Tested applicationsSuitable for: WB, IP, Flow Cyt, Indirect ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human PLK1.
- WB: 293, HeLaS3 or U2OS cell lysate ICC: HeLaS3, NIH 3T3 or U2OS cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
Our Abpromise guarantee covers the use of ab178666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Indirect ELISA||Use at an assay dependent concentration.|
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
Tissue specificityPlacenta and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
- Information by UniProt
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
This flow cytometry data was generated using the same antibody clone in a different buffer formulation (ab17057).
Overlay histogram showing U20S cells stained with ab17057 (red line). The cells were fixed with 80% methanol (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab17057, 1 µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 1 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
ab178666 has been referenced in 6 publications.
- Liccardi G et al. RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell 73:413-428.e7 (2019). PubMed: 30598363
- Ward A et al. Aberrant methylation of Polo-like kinase CpG islands in Plk4 heterozygous mice. BMC Cancer 11:71 (2011). WB ; Mouse . PubMed: 21324136
- Eot-Houllier G et al. Plk1 regulates both ASAP localization and its role in spindle pole integrity. J Biol Chem 285:29556-68 (2010). WB, ICC/IF ; Human . PubMed: 20615875
- Shang ZF et al. Inactivation of DNA-dependent protein kinase leads to spindle disruption and mitotic catastrophe with attenuated checkpoint protein 2 Phosphorylation in response to DNA damage. Cancer Res 70:3657-66 (2010). WB ; Human . PubMed: 20406977
- Watanabe N et al. Deficiency in chromosome congression by the inhibition of Plk1 polo box domain-dependent recognition. J Biol Chem 284:2344-53 (2009). ICC/IF ; Human . PubMed: 19033445
- Yamaguchi T et al. Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis. J Cell Biol 171:431-6 (2005). PubMed: 16260496