Key features and details
- Chicken polyclonal to PLK1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PLK1 antibody
See all PLK1 primary antibodies
DescriptionChicken polyclonal to PLK1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: HEK-293T PLK1 overexpressing lysate; HEK-293T and K562 cell lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500. Predicted molecular weight: 68 kDa.|
FunctionSerine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.
Tissue specificityPlacenta and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
Developmental stageAccumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
- Information by UniProt
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PLK1 (green) using ab235193 at 5 μg/ml in ICC/IF. Donkey Anti-Chicken IgG DyLight™ 488 Preadsorbed was used as the secondary antibody. Nuclear counterstain was DAPI.
Expected localization: Intracellular.
B) Primary + Secondary incubation
C) Merge of A+B
D) Secondary only
All lanes : Anti-PLK1 antibody (ab235193) at 1/500 dilution
Lane 1 : Opal pre-stained ladder
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) PLK overexpressing lysate
Lane 3 : HEK-293T cell lysate
Lane 4 : Human testis lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
All lanes : Goat Anti-Chicken at 1/40000 dilution
Predicted band size: 68 kDa
Observed band size: 135, 75 kDa why is the actual band size different from the predicted?
Exposure time: 7 seconds
In some lysates 63 kDa band is present.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235193 has not yet been referenced specifically in any publications.