Anti-PLK1 antibody [AZ44] (ab12212)

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Western blot - Anti-PLK1 antibody [AZ44] (ab12212)

    Key features and details

    • Mouse monoclonal [AZ44] to PLK1
    • Suitable for: WB
    • Reacts with: Human, Xenopus laevis
    • Isotype: IgG1

    Overview

    • Product name

      Anti-PLK1 antibody [AZ44]
      See all PLK1 primary antibodies

    • Description

      Mouse monoclonal [AZ44] to PLK1

    • Host species

      Mouse

    • Specificity

      This is a useful antibody to check that extracts have maintained their CSF status and haven't been activated into interphase.

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Recombinant full length protein (Xenopus laevis).

    • Epitope

      This antibody is thought to bind to a phosphorylated epitope. However, this has not yet been proved, but it does stain in a mitotic specific manner.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab12212 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 68 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 68 kDa).

    Target

    • Function

      Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.

    • Tissue specificity

      Placenta and colon.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
      Contains 2 POLO box domains.
      Contains 1 protein kinase domain.

    • Developmental stage

      Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

    • Post-translational
      modifications

      Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
      Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.

    • Cellular localization

      Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell cycle regulated protein kinase antibody
        • PLK 1 antibody
        • PLK antibody
        • PLK-1 antibody
        • plk1 antibody
        • PLK1_HUMAN antibody
        • Polo like kinase 1 antibody
        • Polo-like kinase 1 antibody
        • Serine/threonine protein kinase 13 antibody
        • Serine/threonine protein kinase PLK1 antibody
        • Serine/threonine-protein kinase 13 antibody
        • Serine/threonine-protein kinase PLK1 antibody
        • STPK 13 antibody
        • STPK13 antibody
        see all

      Images

      • Western blot - Anti-PLK1 antibody [AZ44] (ab12212)
        Western blot - Anti-PLK1 antibody [AZ44] (ab12212)This image is courtesy of Julian Gannon, CRUK

        Western blot using ab12212.

        Lane 1: Mitotic CSF Xenopus egg extract
        Lane 2: Interphase Xenopus egg extract
        Lane 3: HeLa cell extract

        AZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.

        Western blot using ab12212.

        Lane 1: Mitotic CSF Xenopus egg extract
        Lane 2: Interphase Xenopus egg extract
        Lane 3: HeLa cell extract
        AZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.

      References (2)

      Publishing research using ab12212? Please let us know so that we can cite the reference in this datasheet.

      ab12212 has been referenced in 2 publications.

      • Esmaili AM  et al. Regulation of the ATM-activator protein Aven by CRM1-dependent nuclear export. Cell Cycle 9:3913-20 (2010). WB ; Xenopus laevis . PubMed: 20935510
      • Wu JQ  et al. PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylation. Nat Cell Biol 11:644-51 (2009). WB ; Xenopus laevis . PubMed: 19396163

      Customer reviews and Q&As

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      Western blot abreview for Anti-PLK1 antibody [AZ44]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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      Abcam user community

      Verified customer

      Submitted Jul 11 2005

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