Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PLK1, phosphorylated at S137.
(Peptide available as ab25712.)
Our Abpromise guarantee covers the use of ab21738 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ICC/IF image of ab21738 stained MCF7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21738, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab21738 has not yet been referenced specifically in any publications.